Optimizing Rooting of White Potato Cuttings: A Study of Three Growth Conditions | InformativeBD

Dennis A. Apuan, from the institute of Philippines. Joevil C. Pepania, from the institute of Philippines. Mark Anthony M. Bactong, from the institute of Philippines  and Angela Katrina M. Dongdong, from the institute of Philippines. wrote a Research Article about, Optimizing Rooting of White Potato Cuttings: A Study of Three Growth Conditions. Entitled, Rooting response of white Potato (Solanun tuberosum L.) stem cuttings under three different conditions. This research paper published by the International Journal of Biosciences (IJB). an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences | INNSpub. an open access multidisciplinary research journal publisher.

Abstract

The need to produce a cheap alternative and farmer level technology in the production of clean planting materials of White Potato (Solanum tuberosum L.) prompted the investigation on the rooting response of clones from stem cuttings of different age and number of nodes under different concentrations of synthetic plant hormone Alpha-Naphthalene Acetic Acid (ANAA). A zero generation (G0) mother plant was used as a source of clones to examine whether it could produce roots in a sterilized medium. In a replicated split-split plot experimental design with three factors such as the age of the mother plant, number of nodes and levels of growth regulator, we found that roots emerged from clones 18 days after planting in a sterilized river sand. Significant effect on rooting was influenced by the age of cuttings (p=0.0058), number of nodes (p=0.0058) and ANAA (p=<0.0001). Moreover, significant interactions were found among age of cuttings, number of nodes and ANAA concentrations on rooting (p=0.0044). Implications for the feasibility of mass producing clean planting materials from cloning G0 mother plant are discussed.

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Introduction

White potato (Solanum tuberosum L.) is a perennial crop belonging to the Solanaceae family grown mainly for its tubers (Spooner et al., 2014). First cultivated 8000 years ago by the Peruvian farmers in Peru’s Central Andes; now it has more than 4000 different cultivars grown globally (Niekerk et al., 2016; Lutaladio et al., 2009). It was initially introduced in Europe in the sixteenth century and was subsequently distributed throughout the world. Potatoes are the world’s primary non-grain staple food in several countries in Europe and some parts of America due to its nutrient content; with China, India, Ukraine and Russia as top producers (Lutaladio et al., 2009; Shahbandeh, 2022).

The biggest obstacle of the white potato industry in Asia, particularly Philippines is the source of clean planting materials, because potatoes are attacked by bacterial wilt disease caused by pathogen Ralstonia solanacearum. The conventional method of white potato propagation is through the use of tubers, but the risk is high. Other methods are the use of True Potato Seeds (TPS), and through stem cuttings (Morais et al., 2018; Shiwani et al., 2021). At present, the Department of Agriculture’s Northern Mindanao Agri Crops and Livestock Research Complex (DANMACLRC) uses the tissue culture technology to mass produce seedlings and tubers as the source of potato clean planting materials, but cannot cope up with the current demand; thus there is pressure to explore other methods.

Numerous studies were conducted to explore and enhance the propagation of potato through stem cuttings. The work of Zaki and Moustafa (2018) for example, used Indole Acetic Acid (IAA) and Indole Butyric Acid (IBA) at higher concentrations reaching up to 6000 parts per million (ppm) but rooting responses of potato varieties tested differ significantly. Ezzat (2016) dipped the stem cuttings for 1 minute to various rooting hormones such as Indole-3-butyric acid Potassium salt (K-IBA) at 1000 ppm, IAA at 250, and 1-Naphthaleneacetic acid NAA at 500 mg/L. The same hormone IAA was tested by Nikmatullah et al. (2018), but other factors such as age of mother plant and number of nodes was included.

In this study we explored the responses and interactions at different ages of Granola white potato stem cuttings, number of nodes, and levels of hormones in terms of its rooting ability and growth. Results and its potential for tuber production are discussed.

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Source : Rooting response of white Potato (Solanun tuberosum L.) stem cuttings under three different conditions