Foliar Glycine Betaine Improves Broccoli Performance | InformativeBD

Effect of foliar applied glycine betaine on growth performance of broccoli (Brassica oleracea var. Italica)

Sana Liaqat,  Nida Mansoora,  Muhammad Shahnawaz Bhatti,  Shafaq Aslam,  Nida Batool, Sana Ishaq, Ashir Masroor, and Filza Ghafoor, from the institute of Pakistan. wrote a Research article about, Foliar Glycine Betaine Improves Broccoli Performance. Entitled, Effect of foliar applied glycine betaine on growth performance of broccoli (Brassica oleracea var. Italica). This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Glycine betaine performs a crucial role, like osmoprotectant and cytoplasmic osmotic solute specifically in the members of family Gramineae and Chenopodiaceae. Brassica oleracea var. Italica subvariety PALMIRA 2 hybrid due to the presence of glucosinolate plays a vital role for humans. A pot experiment was performed at the Old Botanical Garden, University of Agriculture, Faisalabad. 35 days seedlings were transplanted to pots filled with 7 kg of soil for two weeks to get them acclimatized to pot soil conditions. Foliar application of glycine betaine (0, water spray, 5, 10, and 15 mM) was given after 2 months of transplantation. Data regarding the growth parameters, reactive oxygen species, antioxidant enzymes, mineral ions determination, nutrient use efficiency, nutrient uptake, total soluble proteins, and photosynthetic pigments were collected after 15 days of glycine betaine application. Application of GB increased shoot fresh and dry weight, shoot length, root dry weight, shoot calcium, potassium, nutrient uptake of sodium, calcium, potassium, SOD, peroxidase, and total soluble proteins of Broccoli (Brassica oleracea var. Italica). Implementation of GB showed non-significant results of parameters such as root fresh weight, photosynthetic pigments (chlorophyll a, b, a/b, carotenoids, and total chlorophyll), nutrient use efficiency of sodium, and catalase activity.

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Introduction

Glycine betaine performs a crucial role, like osmoprotectant or as a well-suited cytoplasmic osmotic solute specifically in the members of family Gramineae and Chenopodiaceae. Glycine betaine is zwitterionic, N-methyl derivative present in many higher plants, microorganisms, and animals (Saeed et al., 2016). In plants improved the action of antioxidants, proteins, enzymes, and photosynthetic activity of plants (Banu et al., 2009).

Synthesis of glycine betaine is not the same in different plants for example some plants like barley and spinach gather comparatively high levels of GB as compared to tobacco and Arabidopsis thaliana in their chloroplasts. Application of glycine betaine protect plants from stress, and it is introduced in higher plant and microorganisms by genetic engineering which shows the importance of GB.

It keeps the cell membrane integral in its nature and enzyme equilibrium takes part in the removal of ROS from the cell (Sakamoto and Murata, 2002).

In agriculture from an economical and defensive perspective, foliar-applied glycine betaine is very important. Due to its ameliorative nature to enhance crop production and reduces toxic environmental stresses. Besides GB improves the activities of antioxidant enzymes in rice seedlings. Accumulation of glycine betaine protects plant plasma membrane from disruption, high temperature (Hasanuzzaman et al., 2014).

Broccoli variety PAALMIRA 2 hybrid member of Brassicaceae family and grows up to 90cm in length. It is found around the Mediterranean region and southwestern Europe. It is an old vegetable native to Turkey and its cultivation starts there, later it is found in the USA, England.

In 1923 its industrial cultivation started (Decoteau, 2000). Broccoli is composed of sulforaphane, glucosinolates, selenium, polyphenols, and secondary metabolites, A, C, and E vitamins are also present.

Isothiocyanates present in broccoli, help in the prevention of cancer, its leaves are also utilized in the treatment of skin-related diseases. The presence of glucoraphanin sulforaphane in broccoli high concentration completely hinder the chemical induction of breast cancer in rats (Meyer et al., 2008).

The sprouts of broccoli are famous because of rich in vitamin contents, minerals, and glucosinolate secondary metabolites act as phenolic compounds (Baenas et al., 2012).

 Objectives To evaluate the effect of foliar application of glycine betaine on broccoli variety PALMIRA 2 hybrid.

To observe the changes in physiological and morphological characters of broccoli by foliar spray of glycine betaine.

Reference

Baenas N, Moreno DA, Viguera G. 2012. Selecting sprouts of Brassicaceae for optimum phytochemical composition. Journal of Agricultural and Food Chemistry 60, 11409–11420.

Banu MN, Hoque MA, Sugimoto MWK, Matsuoka Y, Shimoishi YN, Murata Y. 2009. Proline and glycine betaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress. Journal of Plant Physiology 166, 146-156.

Decoteau D.  2000. Vegetable Crops.  Prentice Hall, Upper Saddle River, New Jersey.

Hasanuzzaman M, Alam MM, Rahman A, Nahar K, Fujita M. 2014. Exogenous Proline and Glycine Betaine Mediated Upregulation of Antioxidant Defense and Glyoxalase Systems Provides Better Protection against Salt-Induced Oxidative Stress in Two Rice (Oryza sativa L.) Varieties. BioMed Research International 16, 4-17.

Jabeen N, Abbas Z, Iqbal M, Rizwan M, Jabbar A, Farid M, Abbas F. 2016. Glycine betaine mediates chromium tolerance in mung bean through lowering of Cr uptake and improved antioxidant system. Archives of Agronomy and Soil Science 62, 648-662.

Khalifa GS, Abdelrassoul M, Hegazi AM, El-Sherif MH. 2016.  Attenuation of negative effects of saline stress in two lettuce cultivars by salicylic acid and glycine betaine. Gesunde Pflanzen 68, 177–189.

Lutts S, Majerus V, Kinet JM. 1999. NaCl effects on proline metabolism in rice (Oryza sativa) seedlings. Plant Physiology 105, 450-458.

Meyer M, Adam ST. 2008. Comparison of glucosinolate levels in commercial broccoli and red cabbage from conventional and ecological farming. European Food Research and Technology 226, 1429-1437.

Rahman MS, Miyake H, Takeoka Y. 2002. Effects of exogenous glycine betaine on growth and ultrastructure of salt-stressed rice seedlings (Oryza sativa L.). Plant Production Science 5, 33-44.

Abbreviations SOD Superoxide dismutase, POD peroxidase. 

Article source : Effect of foliar applied glycine betaine on growth performance of broccoli (Brassica oleraceavar. Italica) 

Farmers and Pesticide Practices in Rawalpindi, Pakistan | InformativeBD

Pesticide usage by Farmers; A case study of District Rawalpindi, Pakistan

Antonio-Abdu Sami M. Magomnang, from the institute of Philippines and Dianne Mae M. Asiñero, from the institute of Philippines. wrote a Research article about, Farmers and Pesticide Practices in Rawalpindi, Pakistan. entitled, Pesticide usage by Farmers; A case study of District Rawalpindi, Pakistan. This research paper published by the International Journal of Agronomy and Agricultural Research | IJAAR. an open access scholarly research journal on Agronomy. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

As well pesticides are playing an important role in enhancing crop yield, but producing resistance against many crop pests and are also harmful to our environment. Surveys were conducted in 21 villages of district Rawalpindi, Pakistan in 2016. Data about pesticide usage were collected from 210 respondents (local farmers). The results show that only 22% local farmers have potential towards use of pesticides. The maximum pesticide users were found in Taxila and the price was the key factor to select pesticides for the farmers. Selection of pesticide maximum depends on previous experience of farmers. Only 3 % farmers adopt precautionary measures before using the pesticides in this area. This study will be helpful in future to aware local people about the safe and accurate use of pesticide to produce healthy and good yield crops and also helpful to conduct awareness programs in the future.

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Read more : Bacterial Wilt Threat:Ralstonia solanacearum Impact on Crops in South Kalimantan | InformativeBD 

 Introduction

The Philippine agricultural sector has depended and relied on inorganic fertilizers and pesticides for food production for almost over three decades. Due to lack of an effective and locally available fertilizer and pesticide technologies, Philippines resorted to importation of 85% of its total inorganic fertilizer and more than 90% of its pesticide requirements. A study conducted by Javier and Brown (2014) revealed that large amounts of foreign exchange spent on importation have contributed to the stagnant and limited growth of our economy. Hence, bio-fertilizer research in the country was undertaken in the late 70s to come up with more cost-efficient local alternatives to imported fertilizers and pesticides.

Organic agriculture was developed not just to aid the limited economic growth of the country but also to help poor farmers who cannot afford the insurmountable rising cost of inorganic fertilizers. Organic agriculture is a specific type of low external input agriculture that adheres to certain principles in the production and transformation of agricultural commodities (Pendar, 2008). It is based on minimizing the use of external inputs and avoiding the use of synthetic fertilizers and pesticides (WHO, 2001). Organic farming has grown rapidly in the past few decades, especially in industrialized nations, and organic products were one of the most rapidly growing segments of the retail food industry in these countries. In 2004, some 24 million hectares were globally managed organically in three countries (Argentina, Australia and Italy), and much of this farming involved an extensive, organically certified grazing land (Yussefi, 2004).

An Executive Order 481 was signed by President Gloria Macapagal-Arroyo of the Philippines on December 27, 2005 on the Promotion and Development of Organic Agriculture in the Philippines. Then, Agriculture Secretary Domingo F. Panganiban during this time signed an Administrative Order No. 9 series of 2006 or the Implementing Rules and Regulations (IRR) of EO 481. With this development, the Department of Agriculture has come up with programs and projects in support to EO 481(ATI, 2006).

To promote organic farming in the country, this study attempted to utilize natural farming through the application of Indigenous Microorganism (IMO) in growing a particular plant. IMO has been successfully tried by government agriculturists, academic researchers, non-profit organizations and farmers alike. Studies have found that IMO is useful in removing bad odors from animal wastes, hastening composting, and contributing to crops’ general health (Business Diary, 2013).

The main purpose of this study is to grow Brassica juncea (lettuce) plant using organic fertilizers developed from the species of Paspalum conjugatum (Carabao grass) and Cynodon dactylon (Bermuda grass). Prior to determining the effects of this developed organic fertilizers, the soil samples used were determine in terms of size of particles to examine permeability and capillary of water; pH (acidity or basicity) and the nutrient contents of the soil (Nitrogen, Potassium and Phosphorus).

Reference

Anwar T, Ahmed I, Seema T. 2011. Determination of pesticide residues in fruits of Nawabshah District, Sindh, Pakistan. Pak. J. Bot., 43(2), 1133-1139.

Basa CH, Gregorcic A, Velikonja BS, Kmecl V. 2007. Pesticide residues in agricultural produce of Slovene origin in the period from 2001 to 2005. Acta alimentaria, 36(2), 269-282.

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Parveen Z, Masud SZ. 2003. Monitoring of pesticide residues in human milk. Pak. J Sci. Ind Res., 46, 43-46.

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Khan  MS. 1998. Pakistan crop protection market. PAPA bulletin, Volume 9, 7- 9.

Khan IAT0, Parveen Z, Riazuddin, Ahmed M. 2007. Multi-residue determination of organophosphorus pesticides and synethetic pyrethroids in wheat. Int. J. Agric. Biol., 6(9), 905-908.

Article source : Pesticide usage by Farmers; A case study of District Rawalpindi, Pakistan 

Bacterial Wilt Threat: Ralstonia solanacearum Impact on Crops in South Kalimantan | InformativeBD

Intensity of Ralstonia solanacearum bacterial cause wilting disease in several plants in South Kalimantan, IndonesiaYusriadi, from the institute of Indonesia. wrote a Research article about, Bacterial Wilt Threat: Ralstonia solanacearum Impact on Crops in South Kalimantan. Entitled, Intensity of Ralstonia solanacearum bacterial cause wilting disease in several plants in South Kalimantan, Indonesia. This research paper published by the Journal of Biodiversity and Environmental Sciences | JBES. an open access scholarly research journal on Biodiversity. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Disease that develops and is very detrimental to plants in South Kalimantan is a bacterial wilt disease and has spread in plantations which are superior commodities, with high attack rates. Since 2007 losses due to this disease have increased until mid-2017 losses of up to 80% (severe attack rate). The cause of this disease is the bacterium Ralstonia solanacearum, which is found in almost all of Indonesia. This bacterium is the most important limiting factor for the successful production of 33 families or 150 types of plants both cultivated plants and ornamental plants, such as tomatoes, peanuts, ginger, bananas, eggplant, potatoes and tobacco which are dangerous diseases in the subtropics and tropics because this bacterium has many host plants. This study aims to determine the level of attack on several plants that are widely cultivated in South Kalimantan. This level of attack is very important for a comprehensive alternative control. The method used was sampling at each planting location and sampling in the affected areas of the plantations, then identification was carried out. The results showed that there was no difference in the level of attack on crops caused by these bacteria obtained from the South Kalimantan area of ​​Indonesia.

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Read more : Liver Shield from Nature: Hepatoprotective Potential of Berberis lycium Extracts | InformativeBD

Introduction

South Kalimantan in the development of the agricultural sector is felt to be very potential, this is supported by the vast land conditions, and sufficient labor available and conducive security conditions. Various policies and strategic steps in accelerating development in this field, the provincial government of South Kalimantan through the Department of Agriculture, Food Crops and Horticulture carries out policies and activities in the framework of Safeguarding Food Security through policies a). Maintaining/increasing the surplus of rice production, b) Increasing the availability of other food (crops and horticulture). Policy in increasing production, productivity, competitiveness and value added of food crops and horticulture products (TPH) by focusing on the development of superior commodities (Rice, Corn, Peanuts, Oranges, Bananas and Rhizomes) with the commodity zoning approach, as well as optimizing the control of disturbing organisms plants (OPT) and anticipation of natural disasters on agricultural land.

In Indonesia wilting caused by bacteria is a disease that is very detrimental to cropping. In general, bacterial wilt caused by Ralstonia solanacearum (Yabuuchi et al., 1995) is one of the main obstacles in the production of almost all types of plants both in tropical and temperate climates. It is estimated that there are 50 plant families including Solanaceae, Musaceae, Asteracea, Fabaceae, including families of forest trees, shrubs and weeds. The cause of the bacterial wilt disease of R. solanacearum is known to have a wide geographical distribution and diversity of races (strains) so to control it first needs to know its races, as well as its biovar and host. So far, wilted bacteria are divided into biovar groups (Hartman, Hong, Hanudin & Hayward, 1993) and racial groups, each of which is differentiated based on phenotypic characteristics and host range.

Bacterial wilt caused by bacteria (Rasltonia solanacearum) is one of the main diseases and is widespread especially in plants that have economic value such as potatoes, tomatoes, eggplant, peppers, ginger, chillies, peanuts, bananas and tobacco in tropical and subtropical regions (Hayward 1990; Hayward 1994). Machmud, (1989) that weed species found in peanut fields are also potential hosts for P. solanacearum bacteria. The role of weeds as an important source of inoculum, because bacteria that attack weeds sometimes do not show real symptoms. These bacteria have many races and different levels of attack on different plants, as well as in different regions will be found different races are not the same and sometimes have distinctive characteristics (both biology and physiology). This research will aim to look at the level of attack and spread of these bacteria, so that we will get a type of plant that is safe to be cultivated in certain areas, as well as integrated control tactics that will be prepared to prevent this wilt attack. In South Kalimantan the bacterial wilt of R. solanacearum is a major limiting factor in the production of Kepok bananas (2007 to present) Yusriadi et al., 2017). But lately it turns out that wilting is not only found in banana plants, but has been found in vegetable and other horticultural crops. This study aims to determine the level of attack on several vegetable and horticultural crops that are widely cultivated in South Kalimantan, carried out an overall alternative control.

Reference

Aley EBFLGP, Elphinstone J. 1995. Culture Media For Ralstonia solanacearum Isolation, Identification and Maintenance. Fitopatologia 30(3), 126-130.

Buddenhagen IW. 1986. Bacterial wilt revisited. Proc. Int. Workshop, PCARRD pp. 126-143, 8-10 Oct. 1986. ACIAR Proceeding. Los Banos, Philippines

Djaya AA. 1994. Upaya Pengendalian Layu Bakteri (Pseudomonas solanacearum E. F. Smith) Pada Jahe dengan Mikroorganisme Antagonis, Perlakuan Bibit dan Tanah. Tesis Prog. Pascasarjana IPB, Bogor. 74 hal.

Fahy EM, Persley GJ. 1983. Plant Bacterial Disease a Diagnostic Guide. Academic Press. Australia. 303p.

Hartman GL, Hong WF, Hanudin & Hayward AC. 1993. Potensial of Biological and Chemical Control of Bacterial Wilt. In Hartman GL & Hayward AC (eds). Bacterial Wilt. Proc. of an international conference held at Kaohsiung, Taiwan, October 1992. ACIAR Proceedings .

Hayward AC. 1991. Biology and epidemiologi of bacterial Wilt caused by Pseudomonas solanacearum. Annu. Rev. Phytopathol 29, 65-87.

Hayward AC. 1994. The host of Pseudomonas solanacearum. In Hayward, A.C. & G.L. Hartman (eds.). Bacterial Wilt, the Disease and its Causative Agent P. solanacearum. CAB Int UK 9-24.

Hayward AC. 1990. Diagnosis, Distribution and Status of Groundnut Bacterial Wilt. In Middleton & Hayward (eds.). Proceeding of an ACIAR/ICRISAT collaborative research planning meeting held at Genting Highlands, Malaysia 1990. ACIAR Proceedings 31, 12-17.

Horita M, Tsuchiya K. 2001. Genetic Diversity of Japanese Strain of Ralstonia solanacearum. APS Press. 91(4), 399-407.

Kumar A, Sarma YR, Anandaraj M. 2004. Evaluation of genetic diversity of Ralstonia solanacearum causing bacterial wilt of ginger using REP-PCR and PCR-RFLP. Current Science 87(11), p-1555-1561.

Machmud M. 1989. Resistensi Varietas dan Plasma Nutfah Kacang Tanah terhadap Penyakit Layu (Pseudomonas solanacearum). dalam Syam, Mahyudin (Penyunting) Sem. Hasil Penelitian Tanaman Pangan Bogor 471-482.

Machmud M. 1986. Bacterial wilt in Indonesia. In Persley G.J. (ed). Bacterial Wilt Disease in Asia and the South Pacific. Proc. Of an Int. Workshop held at PCARRD-ACIAR, Philippines. ACIAR Proceedings No. 13, 32-34.

Mairawita Habazar T, Hasyim A, Nasir N, Suswati. 2012. Potensi Serangga Pengunjunga Bunga Sebagai Vektor Penyakit Darah Bakteri (Ralstonia solanacearum Phylotipe IV) Pada Pisang di Sumatera Barat. J. Ento. Indonesia 9(1), 38-47.

Suryadi Y., M. Machmud. 2004. Kemajuan Teknik Deteksi dan Identifikasi Pseudomonas solanacearum.Jurnal Tinjauan Ilmiah Riset Biologi dan Bioteknologi PertanianVolume 1 Nomor 1. Bogor.

Suryadi Y., Rais SA. 2009. Respon Beberapa Genotipe Kacang Tanah Terhadap Penyakit Layu Bakteri (Ralstonia solanacearum) di Rumah Kaca. Bul. Plasma Nutfah 15(1), 20-26.

Yabuuchi E, Osaka Y, Yano I, Hotta H, Nishiuchi Y. 1995. Transfer of two Burkholderia and Alcali Genes Spesies to Ralstonia Gen; Proposal of Ralstonia picketti (ralston, palleroni and Doudroff, 1973) Comb., Ralstonia solanacearum (Smith, 1986) Comb. Nov. and Ralstoni aeutropha (Davis, 1969) Com. Nov.

Yusriadi. 2010. Karakteristik Bakteri Ralstonia solanacearum Penyebab Penyakit Layu Tanaman Tomat di Banjarbaru. J. Chlorophyl 6(3), 137-141.

Yusriadi, A. L. Abadi, S. Djauhari, H. Halim. 2012. Keragaman Bakteri Ralstonia solanacearum Penyebab Penyakit Layu Pisang Di Kalimantan Selatan. Prosiding Seminar Nasional Biodevirsitas IV Departemen Biologi Univ. Airlangga.

Yusriadi, A, L. Abadi, S. Djauhari, H. Halim. 2017. Distribution and Diversity Ralstonia solanacearum wilt disease bacterial causes of banana (Kepok: Local Indonesia) and intensity of attack in South Kalimantan, Indonesia. Journal of Biodiv. and Environ Sci. (JBES) 11(2), p. 78-83.

Yusriadi, Tjahjono B, Sinaga MS, Machmud M. 1998. Pengaruh Pemberian Mikoorganisme Antagonis (P. fluorescens & Trichoderma spp.) terhadap perkembangan Penyakit Layu Bakteri (P. solanacearum E.F. Smith) tanaman kacang tanah. Buletin HPT IPB 9(2). 

Article source : Intensity of Ralstonia solanacearum bacterial cause wilting disease in several plants in South Kalimantan, Indonesia  

Liver Shield from Nature: Hepatoprotective Potential of Berberis lycium Extracts | InformativeBD

Evaluation of hepato-protective activity of B. lycium methenolic crude extracts collected from Distric Sherani, Balochistan

Sami Ullah Sherani,  Javied Iqbal,  Amanullah Khan,  Muhammad Ali Khan, Nadeem Rashid,  Mohammad Rahim Niazi,  Zia Ud Din and Mohamad Kamran Taj, from the institute of Pakistan. wrote a Research article about, Liver Shield from Nature: Hepatoprotective Potential of Berberis lycium Extracts. entitled, Evaluation of hepato-protective activity of B. lycium methenolic crude extracts collected from Distric Sherani, Balochistan. This research paper published by the Journal of Biodiversity and Environmental Sciences | JBES. an open access scholarly research journal on Biodiversity. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Berberis lycium which is commonly called as Indian barberry (English) and Kashmal or Ishkeen in Urdu, is a spiky plant which is the member of the genus Berberis of family Berberidaceae. B. lycium also includes anti-hepatotoxicity effect, when was mixed with G. aparine and P. integerrima and was tested in rats that were treated with carbon tetra chloride; the results revealed that the combination of these three medicinal plants encompasses anti hepatotoxicity effects. B. lycium (root) sample was collected from the hill of village of Ahmadedergah near to Tahkhta Suleiman District Sherani Balochistan, Pakistan. The collected samples were identified by Pharmacognosy, Department faculty of Pharmacy University of Baluchistan, Quetta. Adult healthy rabbits (male) having weights approximately 950g-1300g, were kept in animals house at CASVAB, UoB. Sample serum, within 3 hours after collection , was analyzed  for certain biochemical parameters (Alkaline phosphatase (ALP), Alanine aminotransferase (ALT), Gamma glutamyl transpeptidase (γ-GT), Aspartate aminotransferase (AST), and total Proteins like Bilirubin,  Albumin and Globulin) through automatic analyzer (Merck) at 37oC through standard reagent kits. The obtained values for LFT in the current portion of controlled group were, 0.76± 0.060, 0.032±0.008(mg/dL), 4.2±0.802, 93.8±1.500, 2.28±0.107 and 273±2.818(U/L) for TB, DB, ALT, AKP, GGT, SGOT and SGPT (AST) levels. Whereas, the group treated with CCl4 these parameters were, 0.86±0.075, 0.064+0.006(mg/dL), 4.6±0.680, 2.44±0.150, 90.4±3.467 and 218.4±1.439(U/L) level. While, the group treated with CCl4 and B. lycium 500mg, the above parameters were calculated as 0.92±0.086, 0.442±0.228(mg/dL), 4.4±0.601, 93.2±1.244, 2.62±0.097 and 221.6±1.540(U/L), respectively.

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Introduction

In Pakistan, many native plants are utilized in herbal medicine to treat diseases and injuries. Such plants often shows a broad spectrum of biologic and pharmacologic activities, for example, they are able to reduce inflammation, having antibacterial and antifungal properties Cowan MM. “(1999). The root, bark, seed and fruit extracts of the medicinal plants are used in syrups and infusions, traditionally (Imtiaz and Manzoor, 2003).

Berberis lycium, which is commonly called as Indian barberry (English) and Kashmal or Ishkeen in Urdu, is a spiky plant which is the member of the genus Berberis of family Berberidaceae. (Sabir et al., 2013). It is found in the moderate and semitropical Asian, European and American divisions (Jabeen et al., 2015). In Pakistan it is extensively distributed in Baluchistan, KPK and Punjab provinces, especially in northerly regions, Swat and Azad Kashmir at elevation of 900 to 2900m (Dhar et al., 2012)

B. lycium is a vertical flowering bush plant that increases to a length of 3-4 meters, having a solid timber stem and is enclosed in a slight fragile bark. The branches of B. lycium are light white to grayish and have thorns alternatively fixed on them (Ahmad and Sharif, 2009). Root bark could be approximately 3 mm solid, from the outside fractured and inside smooth (Chauhan, N. S. (1999) B. lycium is extensively utilized for the treatment UTI, swelling of spleen, stomach and intestinal ulcer and liver diseases (Irshad et al., 2013). Local population utilizes the powdered form of dried root bark after combining with dissolved animal fat for bone fractures as a bandage. Shoots of the plant are employed for the abdomen pain, jaundice and loose bowels (Beers, 2012). The bark of the plant has wound healing activity (Asif, et al., 2007).

B. lycium also includes anti-hepatotoxicity effect, when was mixed with G. aparine and P. integerrima and was tested in rats that were treated with carbon tetra chloride; the results revealed that the combination of these three medicinal plants encompasses anti hepatotoxicity effects. (Atawodi et al., 2011). To estimate Hepatoprotective effect of B. lycium, crude powder and Methanolic extract of plant were used. CCl4 was given to the rabbits to induce hepatotoxicity. Results showed that plant considerably decreased the elevated levels of serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase and alkaline phosphatase enzymes in hepatotoxic rabbits (Malik et al., 2011). The present study was therefore designed to evaluate the in vitro Hepatoprotective effect of B. lycium.

Reference

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Dhar S. 2012. “In vitro plant regeneration system for Berberis lycium using cotyledonary node explants”. Journal of Tropical Medicinal Plants  13(1), 51-55.

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Article source : Evaluation of hepato-protective activity of B. lycium methenolic crude extracts collected from Distric Sherani, Balochistan 

Pistacia integrrima: Evaluating the Value of an Important Medicinal Plant | InformativeBD

Evaluation of Pistacia integrrima; an important plant

Muhammad Shuaib,  Kashif Ali, Umar Zeb,  Firasat Hussain,  Muhammad Aurang Zeb, Saddam Hussain, and Fida Hussain, from the institute of China. wrote a Research article about, Pistacia integrrima: Evaluating the Value of an Important Medicinal Plant. Entitled, Evaluation of Pistacia integrrima; an important plant. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Pistacia integrrima is a typical therapeutic plant belongs to family Anacardiaceae and local to Japan, China and found in the Northern regions of Pakistan commonly called KakrraSingi (Urdu) and Shanai (Pushto). It is used ethnomedicinally for a number of diseases include fever, cough, asthma, vomiting, Ascaris, Anorexia, Allergy, viral infections, diarrhea, snake and scorpion biting sting. The different parts of the plant roots, leaves, stems, barks, Galls and fruits contains various bioactive compounds like amino acid, alkaloids, sterols, flavonoids, tannins, saponins, aromadendrene, Pistacinin, Pistacin, Dihydromalvic acid, Pistacienoic acid, sitosterol, resin, essential oils, caprylic acid, camphene, cineol, sterals, dihydroqueretin and triterpenoids. Antimicrobial activity of chloroform and ethanol leaves extract from Pistacia integrrima reported in many research papers. The leave extract exhibited the fungi growth including Aspergillus flavus, Dreschlera turcica and Fusarium verticillioides. The plant is known to have many biological activities including antibacterial, antifungal, analgesic, antioxidant, phytotoxic, cytotoxicity and antiasthmatic. The current review will cover biological activities, phytochemical evaluation, ethnomedicinal uses, ethnobotanical uses and aim to serve as a base for additional investigation and exploitation. The current review shows a gap needed further investigations and isolations of new compound, and its biological activities.

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Introduction

Pistacia integerrima belong to family Anacardiaceae and a native dioecious tree to China, Japan, Pakistan, Afghanistan, and India (Pant and Samant, 2010). The different researcher goal medicinal flora like the development of therapeutic compounds (Elisabetsky, 1991).

There is some disease in the world which cause much death killing almost 40000 people, a disease like diarrhea cause huge mortality among children’s (Piddock et al., 1991). Bacteria like Escherichia coli, Salmonella spp. and Staphylococcus aureus are most common species which are pathogenic to children (Singh 1992). In recent years drug resistance to human pathogenic bacteria has been commonly reported from all over the world (Mulligen et al., 1992). Plant-based drugs are 120 worldwide and it is obtained from 95 plants.

About 250,000 flowering species and about 5000 flowers had pharmaceutical potential assessed. In East Asia, many plants are considered to have significant medicinal features i.e. antiinflammatory, anti-bacterial and analgesic functions because they contain a large variety of phytochemical i.e. monoterpenoids, sesquiterpenoids, and curcuminoids (Tang, 1992).

It is found and mostly grows at an altitude of 900- 2000m. Pistacia integrrima commonly called zebra wood but it has many vernacular names in Pakistan like Shania, Kakra, Khanjar, Thoak and India like Kakring, Kakra, Kakroi, Kakkar, Singhi, kakarsinghi (Orwa et al., 2009). Pistacia integrrima is a well prominent due to Galls that present on the leaves and petioles. These galls are like animals horn shaped. The galls are the store house of various secondary metabolites so; it has importance in Indian traditional medicine systems (Chopra et al., 1986).

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 Article source : Evaluation of Pistacia integrrima; an important plant 

Palm Weevil Profile: Biology of Rhynchophorus phoenicis in Côte d’Ivoire | InformativeBD

Biological studies on palm tree weevil Rhynchophorus Phoenicis fabricius (Coleoptera; Curculionidae): An interest food bug in Côte d’Ivoire (West Africa)

Ehounou Prisca Gnanda, from the institute of Côte d’Ivoire and  Ouali-N’goran San-Whouly Mauricette, from the institute of Côte d’Ivoire. wrote a Research article about, Palm Weevil Profile: Biology of Rhynchophorus phoenicis in Côte d’Ivoire. Entitled, Biological studies on palm tree weevil Rhynchophorus Phoenicis fabricius (Coleoptera; Curculionidae): An interest food bug in Côte d’Ivoire (West Africa). This research paper published by the International Journal of Biosciences | IJB.  an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Larvae of the palm tree weevil Rhyncophorus phoenicis are consumed and sold on the markets in Côte d’Ivoire, their high prices, in fact a luxury product. In order, to consider possibilities of domestication to increase their availability and decrease the cost, the objective of the present work is to know the development cycle of this species. To do this, a breeding was conducted at the laboratory of Zoology and Animal Biology of the University Felix-Houphouet-Boigny. The rearing device consisted of cylindrical and rectangular plastic boxes. The individuals used come from cocoons collected from palms infested in the field. As soon as the imagoes appeared, pieces of palm trunk were placed in these boxes to serve as support for spawning and nutrition. The total cycle time is 108.51 ± 3.6 days and has 6 larval stages, a nymphal stage followed by adult stage. Female lifetime fecundity was 252.26 ± 3.61 eggs. Incubation period was 4.09 ± 0.53 days with fertility rate of 88.30%. The mean larval and pupal development period were 33, 24 ± 0.9 days and 25.42 ± 2.4 days, respectively. The average emergence rate of adults was 88.30 ± 2.04%. A significant difference was observed in adult life span (F = 28.08, P < 0.05).It is 68.86 ± 3.3 days in males and 54.71 ± 2.7 days in females. This work revealed the possibility of breeding R. phoenicis under controlled conditions. To avoid abusive harvests in already fragile ecosystem, breeding trials on other substrates would be possible.

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Introduction

The consumption of insects is a food practice that extends more and more in the world (FAO, 2013). Many African peoples consume large quantities even if the usually food tends to disappear gradually (van Huis et al., 2013; Halloran et al., 2014). The united Nations Food and Agriculture organization (FAO) promotes since several years the use of insects in food and feed (FAO, 2010). Indeed, the consumption of insects expands, diversifies the diet, and helps prevent nutritional deficiencies (Malaisse, 2004). In West Africa, both termites, locusts, lepidopteran caterpillars and beetles are eaten. The larval and adult stages of R. phoenicis Fabricius (1801) commonly referred to as the caterpillar of the palm tree, are consumed in Côte d'Ivoire (Ouali and Ehounou, 2017). In addition, the commercialization of this species constitutes an important source of income for traders. In most cases, the insects consumed are directly obtained by harvesting or collecting in a natural environment. However, the availability is related to seasonal variations. The farms are still marginal and require a rigorous development to ensure a supply of quality and quantity for nutrition (FAO, 2013). In terms of rearing, insects have higher rates of growth and feed conversion rates and can breed on small spaces with a low impact on the environment (FAO, 2009; van-Huis, 2013). Irregular supply of markets in the larvae makes it difficult to meet demand especially during the dry season. To compensate these ruptures seasonal in supply, it is necessary to produce this insect outside of its natural habitat. The objective of this study is to know some biological parameters of R. phoenicis. Specifically, it will determine incubation period, female fecundity, egg fertility, the survival rate, larval development time, longevity, sex ratio and emergence rate of adults.

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Article source : Biological studies on palm tree weevil Rhynchophorus Phoenicis fabricius (Coleoptera; Curculionidae): An interest food bug in Côte d’Ivoire (West Africa)