Joseph M. Asomaning, from
the institute of Ghana. wrote a Research article about, Enhancing Garcinia kola
Seed Germination Techniques. Entitled, Germination of Garcinia kola (heckel)
seeds in response to seed sectioning, chemical pretreatment and different
temperatures. This research paper published by the Journal of Biodiversity and Environmental Sciences | JBES. an open access scholarly research journal Biodiversity. under
the affiliation of the International Network For Natural Sciences|
INNSpub. an open access multidisciplinary research journal publisher.
Abstract
Garcinia kola Heckel is
a multi-purpose tree widely used in West Africa resulting in its
over-exploitation, the species is extinction-threatened. On farm conservation
through cultivation has been recommended. However, seeds of the species can
take about 18 months to germinate. This study aims at generating information on
how the combinations of the techniques of seed sectioning, chemical treatment
and temperature can be used to enhance germination of the seed. Seed sections
and whole seeds pretreated with dormancy breaking chemicals were germinated on
a gel of 1% water agar at 20, 25, 30 and 35°C. Statistical design used in the
investigation was a completely randomized design in a 5×7×4 factorial
(germination materials × chemical treatments × temperature). Germination data
showed significances (p<0.001) namely: germination materials, germination
temperatures, germination materials x germination temperatures, germination
materials x chemicals, germination temperatures x chemicals.
Read more : Optimizing In Vitro Organogenesis in Sugarcane Using Plant Growth Regulators | InformativeBD
Introduction
Garcinia is a tropical
plant genus including several species in Africa, America and Asia. These
species are commonly useful for many purposes. The seeds of G. kola have
pharmacological uses in treating coughs, throat infections, bronchitis and
hepatitis (Farombi et al., 2005) The seeds which serve as a bitter stimulant
also serve as snake repellent when they are placed round the compound (Nair,
1990). Other medicinal uses include: purgative, antiparasitic, antimicrobial.
The seeds are used to prevent and relieve colic, cure head or chest colds.
This plant has shown
bronchodilator effect (Orie and Ekon 1993), anti-inflammatory, antimicrobial,
antibacterial and antiviral properties (Akoachere et al., 2002). In laboratory
tests, Garcinia kola was found to halt the deadly disease caused by Ebola virus
in its tracks. The virus causes Ebola hemorrhagic fever - an often-fatal condition
(Anonymous, 1999). Compounds from the plant have also proved effective against
some strains of flu, a contagious respiratory disease also commonly known as
influenza (Iwu, 1993). Its by-products are also useful: the wood makes
excellent fuel wood; its dense rounded crown makes it an ideal tree for shade
around homestead; the branches are used as chewing stick because of its bitter
taste and antibacterial activities of its extracts (Taiwo et al., 1999). The
bark of the stem is used in the tanning and dyeing industry (Irvine, 1961).
Because of its high
interest resulting in its overexploitation, Garcinia kola is
extinction-threatened in several West and Central African countries such as
Ivory Coast (FAO (1996), Ghana (Wong, 1997), Congo and Cameroon (Tchatat,
1999). It is therefore useful to undertake on farm conservation by small holder
farmers through agroforestry systems in order to decrease the pressure on wild
population of the species. However, the major difficulty in Garcinia kola
propagation as for several species of Garcinia genus is related to seeds
germination. Due to dormancy in Garcinia, seeds can take as long as 18 months
to germinate (Aduse-Poku et al., 2003). Some studies to investigate seed
germination of some species in the genus Garcinia has been done at the farmer’s
level and under laboratory conditions. These include Garcinia gummi-gutta
(Geeta et al, 2006); Garcinia indica (Malik et al., 2005); Garcinia kola
(Agyili et al., 2007; Kanmegne and Omokolo 2008).
The present work aims
at generating information on how the combination of the techniques of seed
sectioning, chemical treatment and temperature can be used to enhance the
germination of G. kola. The information will be useful in raising large
quantities of seedlings for several farming communities in Ghana who are being
encouraged to plant the species on their cocoa farms as a shade crop and as a
means of conserving the species.
Reference
Aduse-Poku K, Nyinaku
F, Atiase VY, Awuah R, Mensah EO, Nyantakyi D, Owusu HK and Agyenim-Boateng B. 2003.
Improving rural livelihoods within the context of sustainable development: case
study of Goaso forest district. Tropenbos International Ghana, University of
Amsterdam and Institute of Renewable Natural Resources 50 pp.
Agyili J, Sacandé M,
Koffi E and Peprah T. 2007. Improving the collection and germination of
West African Garcinia kola Heckel seeds. New Forests 34, 269-279.
Akoachere JF, Ndip RN,
Chenwi EB, Ndip LM, Njock TE and Anong DN. 2002. Antibacterial effect of
Zingiber officinale and Garcinia kola on respiratory tract pathogens. East
Africa Medical Journal 79(11), 588-592.
Anegbeh PO, Iruka C and
Nkirika C. 2006. Enhancing germination of bitter cola (Garcinia kola)
Heckel: prospects for agroforestry farmers in the Niger Delta. Scientia
Africana 5(1), 1118-1931.
Asomaning JM, Olympio
NS, Sacandé M. 2011. Desiccation sensitivity and germination of
recalcitrant Garcinia kola Heckel seeds. Research Journal of Seed Science 4(1), 15-27.
Asomaning JM. 2009.
Seed desiccation tolerance and germination of seven important forest tree
species in Ghana. Thesis submitted in partial fulfillment of the requirements
for the degree of Doctor of Philosophy. School of Graduate Studies, Department
of Horticulture. Kwame Nkrumah University of Science and Technology (KNUST),
Kumasi, Ghana 1-187 .
Choudhary DK. 1975.
Causes of poor and erratic germination in Atropa belladonna. Planta Med 27, 18-22.
DOI: 10. 1055/s-0028-1097754.
Daws ML, Gaméné SC,
Sacandé M, Pritchard HW, Groot PCG and Hoekstra F. 2004. Desiccation and
storage of Lannea microcarpa seeds from Burkina Faso. pp 32-39.
Eyog-Matig O, Aoudji
AKN, Linsouss C. 2007. Garcinia kola Heckel seeds dormancy-breaking.
Applied Ecology and Environmental Research 5(1), 63-71.
Farombi
EO, Adepoju BF, Ola-Davies OE and Emerole GO. 2005.
Chemoprevention of aflatoxin B1-induced genotoxicity and hepatic oxidative
damage in rats by kolaviron, a natural bioflavonoid of Garcinia kola seeds.
European Journal Cancer Prevention 14 (3), 207-214.
Food and Agriculture
Organization, FAO. 1996. Côte d’Ivoire: Rapport de pays pour la conférence
technique internationale de la FAO sur les ressources phytogénétiques. FAO 75
pp.
Geeta J, Kumar AH,
Balakrishna G, Srinivisa YB. 2006. Production of supernumerary plants from
seed fragments in Garcinia gummi-guta: evolutionary implications of mammalian
frugivory. Current Science 91(3), 372-376.
International Seed
Testing Association, ISTA. 1999. International rules for seed testing.
Supplement to Seed Science and Technology 27, 1-133.
Irvine FR. 1961.
Woody Plants of Ghana with Special Reference to their Uses. Oxford Univ. Press
143-144.
Malik SK, Chaudhury R,
Kalia RK. 2005. Rapid in vitro multiplication and conservation of Garcinia
indica: a tropical medicinal tree species. Scientia Horticulturea 106, 539-553.
Nair PKR. 1990.
The prospects for agroforestry in the tropics. Technical Paper 131. Nairobi,
ICRAF.
Nzegbule E, Mbakwe R. 2001.
Effect of pre-sowing and incubation treatment on germination of Garcinia kola
(Heckel) seeds. Fruits 56(6), 437-442.
Of ori DA, Asomaning
JM, Peprah T, Agyeman VK, Anjarwalla P, Tchoundjeu Z, Mowo JG, Jamnadass R. 2015.
Addressing constraints in propagation of Allanblackia spp. through seed
sectioning and air layering. Journal of Experimental Biology and Agricultural
Sciences 3(1), 89-96.
Orie NN, Ekon EU. 1993.
The bronchodilator effect of Garcinia kola. East Africa Medical
Journal 70(3),143-145.
Rai ND. 2003.
Human use, reproductive ecology and life history of Garcinia gummi-guta : a
non-timber forest product, in the western Gaths, India. Thesis submitted in
partial fulfillment of the requirements for the degree of Doctor of Philosophy.
Pennsylvania State University, Graduate School, Department of Biology 191 pp.
Smith MT, Wang BSP,
Msanga HP. 2002. Dormancy and germination. In: Tropical tree seed manual,
ed. J.A. Vozzo. U.S. Department of Agriculture Forest Science pp. 149-176.
Taiwo O, Xu HX, Lee SE. 1999.
Antibacterial activities of extracts from Nigerian chewing sticks. Phytotherapy
Research 13(8), 675-679.
Tchatat M. 1999. Produits
forestiers autres que le bois d’œuvre (PFAB): place dans l’aménagement durable
des forêts denses humides d’Afrique centrale. Série FORAFRI, document 18.
CIRAD, CIFOR, IRAD & CARPE 103 pp.
Vogel E. de F. 1980.
Seedlings of dicotyledons – Structure, development, types. Descriptions of 150
woody Malesian taxa. Centre for Agricultural Publishing and Documentation.
Wageningen, 1980. ISBN 90‐220‐0696‐4.
Watson L, Dallwitz MJ. 2009.
The families of flowering plants. Guttiferae. Juss. descriptions, illustrations,
identification and information retrieval. Version 19th August 2014.
http://delta-intkey.com.
Wong JLG. 1997.
That State of Ghana’s Forest (1986-1997). Non-timber forest products. Fountain
Renewable Resource Ltd., UK 29 pp.













