Rapid In Vitro Clonal Propagation of Hybrid Muskmelon (Cucumis melo L.) from Seedling Explants | InformativeBD

Hafizur Rahman, M Shahinozzaman , M Rezaul Karim , M Aminul Hoque , M Monzur Hossain , and AKM Rafiul Islam, from the different institute of the Bangladesh. wrote a research article about, Rapid In Vitro Clonal Propagation of Hybrid Muskmelon (Cucumis melo L.) from Seedling Explants. entitled, Rapid in vitro clonal propagation of a hybrid muskmelon (Cucumis melo L.) cultivar from seedling explants. This research paper published by the International Journal of Agronomy and Agricultural Research (IJAAR). an open access scholarly research journal on Agronomy . under the affiliation of the International Network For Natural Sciences | NNSpub. an open access multidisciplinary research journal publisher.

Abstract

An investigation was undertaken to develop a reliable strategy for large scale multiplication of a hybrid cultivar of muskmelon through in vitro clonal propagation ved in MS medium containing 0.5 mg/l GA3. Two explants excised from axenic seedlings were tested for shoot proliferation and leaf node showed better shoot proliferation than shoot tip explants. Maximum shoots (8.22±0.23) per culture were produced from leaf node explants cultured on MS medium augmented with 1.0 mg/l BA. Rooting was found best on 0.1 mg/l NAA containing medium. Rooted plantlets were acclimatized gradually and transferred to the field condition and finally, 82 % plantlets were survived well.

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Introduction

Muskmelon (Cucumis melo L. cv. reticulata) is a popular fruit plant belonging to the family of Cucurbitaceae. It is native to Persia (Iran), Armenia, and adjacent areas on the west and the east and now widely grown in the tropics, subtropics and the temperate regions of the world (Keng and Hoong, 2005). Due to having high fruit value, muskmelon has recently been introduced in Bangladesh (http://www.sdnbd.org/tomato_harvest.htm). This is an annual plant and each plant can produce an average of 5-7 fruits. The fruits being rich in vitamin B, vitamin C, calcium and β-carotene have both edible and medicinal uses also. Traditionally this plant is cultivated by seeds and the commercial growers face several problems such as high market value (150 BDT/seed) of hybrid seeds, low seed germination rate and disease susceptibility.

In this context, in vitro clonal propagation of hybrid plantlets could be an alternative approach for large scale cultivation of muskmelon. Keng and Hoong, (2005) used field grown nodal explants for in vitro propagation of muskmelon while Tarsem et al., (2005) used in vitro derived plantlets for micropropagation of muskmelon mainly with a view to maintain a male sterile line. But the abovementioned reports are not supported enough for large scale clonal propagation of muskmelon as these do not have a clear concept. Moreover, in Bangladesh no report has been made yet on in vitro clonal propagation of this fruit plant. The present study, therefore, describes the first report on rapid multiplication of homogenous plantlets of a hybrid cultivar of muskmelon via in vitro raised seedling explants.

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Source : Rapid invitro clonal propagation of a hybrid muskmelon (Cucumis melo L.)cultivar from seedling explants