Defending Soybean: Screening Kenyan Varieties Against Rust Disease | InformativeBD

H.A. Ogot,  S.A. Okoth,  G.O. Obiero, and J.M. Mahasi,  from the  different institute of Kenya. wrote a Research article about, Defending Soybean: Screening Kenyan Varieties Against Rust Disease. Entitled, Screening of selected kenyan soybean varieties for resistance to Phakopsora pachyrhizi (Soybean rust). This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Soybean (Glycine max (L.) Merrill.) is a highly nutritious plant which plays an important role in the world’s  economy, however soybean rust  disease caused by the fungus Phakopsora pachyrhizi is a major challange to the soybean industry.  The disease among other constraints  has significatly  affected crop yields in most soybean growing countries.  In this study  Seven  varieties of soybean (Nyala, Bossier, SB19, Hill, SB8, Gazelle and TGx1987-32F) commoly  grown by farmers  in Kenya were tested in the green house for resistance to soybean rust.  The varieties TGx1987- 32F and SB8 showed  resistant reactions  characterized by  red brown lesion with low level of disease severity,  low lesion number,  low sporulation level and low area under disease progress curve (AUDPC) value.  The other five varieties; Nyala, Bossier, SB19, Hill and Gazelle showed susceptible  reactions to  soybean rust producing tan lesion with profuse sporulation and high disease severity level. The Soybean varieties with low lesion densities, low disease severity and low sporulation level may be possible sources of rust resistance genes that can be used in breeding programs to produce rust resistant varieties.

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Introduction

The production of soybean in Kenya is affected by numerous biotic and abiotic factors. Some of the constraints include, low yielding varieties, lack of markets, poor agronomic practices, lack of awareness for its potential, competition with other legumes, drought, water logging, and pest and disease attacks (Hartman et al., 2011). Other factors include lack of varieties which are tolerant midseason moisture stress and high yielding varieties tolerant to low phosphorus (FAO, 2005). Among the biotic factors affecting soybean production diseases are of great concern because of their final impact on yield. There are a number of diseases that infect soybean worldwide the most common disease are Anthracnose, bacterial blight, bacterial pustule, soybean rust, bean pod mottle virus, brown stem rot, charcoal rot , frog eye leaf spot, soybean cyst nematode and soybean mosaic virus among others (Ploper,1997).

Soybean rust caused Phakopsora pachyrhizi as been identified among other diseases as the major challenge to soybean production worldwide. Phakopsora pachyrhizi belongs to the fungal phylum Basidiomycota, class Urediniomycetes and order Uredinales, which produce uredinia, on “dome-like” structures that give rise to asexual urediniospores. Hair-like hyaline hyphae called paraphyses grow inside uredinia. Paraphyses and sporophores are base structures for urediniosopore production (Bromfield, 1984). P. meibomiae is less aggressive while P. pachyrhizi is more aggressive and infects over 95 species of plants from more than 42 genera, including soybean and related Glycine species (Bromfield, 1984). The most susceptible host of P. pachyrhizi is kudzu (Pueraria lobata (Wild.) Ohwi), a weed species that is commonly found in the United States of America. Other common hosts are medic (Medicago arborea L.), lupine (Lupinus hirsutus L.), sweet clover (Melilotus officinalis (L.) Lam), vetch (Vicia dasycarpa Ten), common beans (Phaseolus vulgaris L.), lima and butter beans (Phaseolus lunatus L.), pigeonpea (Cajanus cajan (L.) Millsp), garden peas (Pisum sativum L.) and cowpeas (Vigna unguiculata) (Bromfield, 1984). Soybean rust infection process begins in the low to mid-canopy and moves up the plant. The infection process starts with urediniospores germination to produce a single germ tube that grows across the leaf surface, until an appressorium is formed. Penetration of epidermal cells is direct through the cuticle by an appressorial peg (Miles et al., 2005). During the infection process intracellular invasion of the leaf occurs once hyphae are formed within the mesophyll layer. Within 5 to 7 days volcano shaped uredinia with round ostioles are produced which release urediniospores on the abaxial surface completing the asexual reproduction cycle (Goellner et al., 2010).

The rapid spread of the disease in the continent of Africa has led to major decline in soybean yield (Levy, 2005, Oloka et al., 2008). Losses due to soybean rust can be significantly high. In South Africa losses of 10- 80% have been reported and in areas under monocropping system the losses can be as high as 100%. India has experienced losses of 10-90%, Japan 40% and Taiwan has reported losses of 23-90% in (Hartman et al., 1999). It is therefore important that the major production constraints be addressed so as to improve the crop yield to be able to meet the market demands and sustain the production industries. To control the spread of the rust disease chemical fungicides and cultural practices are used howerever the use fungicides to control the disease commercial plantings significantly increases production costs it is therefore not a feasible option in small scale soybean plantings especially in developing countries (Miles et al., 2003). Furthermore the fungicides are expensive and are not very effective at preventing epidemics as Bonde et al., (2006) noted yield losses of up to 50% under severe rust epidemics with chemical control. Other legumes that also form an integral part of the cropping system such as cowpea, pigeon pea and common beans are functional alternative hosts of P. pachyrhizi which makes control a great challenge (Anon, 2007; Slaminko et al., 2008). Cultural practices like destruction of alternate hosts, timely irrigation, early planting and growing early maturing cultivars can also reduce the incidence of the disease (Akinsanmi et al., 2001). However, the rapid spread by wind-borne urediniospores and the large number of host species increases chances of soybean rust survival making cultural practices relatively ineffective (Hartman et al., 2005).

Planting of disease resistant cultivars is the most viable way to manage soybean rust disease. To identify rust resistant cultivars soybean plants must be screened for resistance to diverse pathogen populations (Twizeyimana et al., 2007). This study therefore aims at screening selected soybean varieties commonly grown in Kenya for resistance to soybean rust isolates under green house conditions.

Reference

Akinsanmi  OA, Ladipo JL, Oyekan PO. 2001. First report of soybean rust (Phakopsora pachyrhizi) in Nigeria. Plant Disease 85, 97.

Anon. 2007. Hosts of Phakopsora pachyrhizi, the Casual Organism of Soybean Rust in South America. Japan International Research Center for Agricultural Sciences (JIRCAS) Newsletter for International Collaboration JIRCAS, Tsukuba, Ibaraki, Japan.

Bonde M, Nester S, Austin C. 2006. Evaluation of virulence of Phakopsora pachyrhizi and Phakopsora meibomiae isolates. Plant Disease 90, 708–16.

Bromfield KR. 1984. Soybean rust, Monograph (American Phytopathological Society), 11, American Phytopathological Society. St. Paul, MN.

FAO. 2005. Food and Agricultural Organization. Online http://fao.org/ag

FAO. 2008. Food and Agricultural Organization. Online  http://fao.org/ag

Garcia A, Calvo E, de Souza Kiihl  R, Harada A, Hiromoto D, Vieira L. 2008. Molecular mapping of soybean rust (Phakopsora pachyrhizi) resistance genes: discovery of a novel locus and alleles. Theoretical Applied Genetics 117, 545-553.

Goellner K, Loehrer M, Langenbach C, Conrath U, Koch E, Schaffrath U. 2010. Phakopsora pachyrhizi, the causal agent of Asian soybean rust. Molecular Plant Pathology 11, 169-177.

Hartman GL, Bonde MR, Miles MR, Frederick RD. 2004. Variation of Phakopsora pachyrhizi isolates on soybean. The Proceedings: VII World Soybean Research Conference, Foz do Iguassue, PR, Brazil: 440-446.

Hartman GL, Miles  MR, Frederick  RD. 2005a. Breeding for resistance to soybean rust. Plant Dis. 89, 664–666.

Hartman GL, Miles MR, Frederick RD. 2005b. Historical viewpoint and soybean resistance to soybean rust. In Proceedings of the 2005 Illinois Crop Protection Conference, pp. 16 – 20. Available Online at: www.ipm.uiuc.edu/education/proceedings/index.html

Hartman GL, West ED, Herman TK. 2011. Crops that feed the World Soybean worldwide production, use, and constraints caused by pathogens and pests. Food Security 3, 5-17.

Hartman GL, Sinclair JB, Rupe JC. Eds. 1999. Compendium of soybean diseases (4th ed.). St. Paul: American Phytopathological Society.

JIRCAS. 2016. Laboratory manual for studies on soybean rust resistance available at http://www.jircas.affrc.go.jp/english/manual/soybean_rust/JIRCAS_manual_soybean_rust.pdf

Kumudini S, Prior E, Omielan J, Tollenaar T. 2008. Impact of Phakopsora pachyrhizi infection on soybean leaf photosynthesis and radiation absorption. Crop Science 48, 2343-2350.

Levy C. 2005. Epidemiological and chemical control of soybean rust in southern Africa. American. Phytopathological journal 89(4), 669-674.

Njoroge NJ, Owouche JO, Oyoo ME. 2015. Evaluation of soybean [Glycine max(L.)Merr.] genotypes for  agronomic and quality traits  in Kenya. African Journal of Agricultural  Research 10(12), p 1474- 1479,  http://dx.doi.org/10.5897/AJAR2014.9168

Oloka HK, Tukumahabwa P, Sengooba T,  Shanmagasundram S. 2008. Reaction of exotic soybean germplasm to Phakopsora pachyhrizi in Uganda. Plant Disease 92(11), 1493-1496. http://dx.doi.org/10.1094/PDIS-92-11-1493

Pham TA, Miles MR, Frederick RD, Hill CB, Hartman GL. 2009. Differential responses of resistant soybean entries to isolates of Phakopsora pachyrhizi. Plant Disease 93, 224-228.

Mahasi JM, Vanlauwe B, Mursoy RC, Mbehero P, Mukalama J. 2009. Increasing productivity of soybean in Western Kenya through evaluation and farmers participatory variety selection, pp. 326-334 12th KARI biannual conference, Nairobi, Kenya.

Mahasi JM, Vanlauwe B, Mursoy RC, Mbehero P, Mukalama J. 2011. A sustainable  approach to increased soybean production in western Kenya.  African crop science conference proceedings 10, 111-116.

Miles MR, Morel W, Ray JD, Smith JR, Frederick RD, Hartman GL. 2008. Adult plant evaluation of soybean accessions for resistance to Phakopsora pachyrhizi in the field and greenhouse in Paraguay. Plant Disease 92, 96-102.

Miles  MR, Bonde  MR, Nester SE, Berner DK, Frederick RD, Hartman GL. 2011. Characterizing resistance to  Phakopsora  pachyrhizi in soybean.  Plant Dis. 95, 577-581.

Miles MR, Frederick RD, Hartman GL. 2006. Evaluation of soybean germplasm for Resistance to Phakopsora pachyrhizi. Online. Plant Health Progress http://dx.doi.org/10.1094/PHP-2006-0104-01-RS.

Miles MR, Rosenblatt I, Traynor P, Hartman GL. 2005. Severity assessment for soybean rust. Proceedings of the National Soybean Rust Symposium, Nov. 14-16, 2005, Nashville, TN. Plant Management Network. Online publication.

Miles MR, Frederick RD, Hartman GL. 2003 Soybean rust: is the U.S. crop at risk? APSnet Feature, American Phytopathological Society. Online publication.

Ploper LD. 1997. Evolution, impact and current status of soybean diseases in Argentina. In World Soybean Research Conference V: Proceedings, B. Napompeth (Ed.), Kasetsart  University Press, p 239- 242.

Sharma RC, Duveiller E. 2007. Advancement toward new spot blotch resistant wheat in South Asia. Crop Sci. 47, 961–968. http://dx.doi.org/10.2135/cropsci2006.03.0201

Slaminko TL, Miles MR, Frederick  RD, Bonde MR, Hartman GL. 2008. New legume hosts of Phakopsora pachyrhizi based on greenhouse evaluations. Plant Disease 92, 767-771.

Twizeyimana M, Ojiambo PS, Sonder K, Ikotun T, Hartman GL, Bandyopadhyay R. 2009. Pathogenic variation of Phakopsora pachyrhizi infecting soybean in Nigeria  Phytopathology 99, 353-361.

Twizeyimana M, Ojiambo PS, Ikotun T, Paul C, Hartman GL, Bandyopadhyay R. 2007 Comparison of field, greenhouse, and detached-leaf evaluations of soybean germplasm for resistance to Phakopsora pachyrhizi. Plant Dis. 91, 1161-1169.

Wanderi SW. 2012. Genetic analyses for resistance to soybean rust (Phakopsora pachyrhizi) and yield stability among soybean genotypes in Kenya.  PhD thesis University of KwaZulu-Natal.

Yamanaka N, Yamaoka Y, Kato M, Lemos NG, Passianotto, ALL, Santos JVM, Benitez ER, Abdelnoor RV, Soares  RM,  Suenaga K. 2010. Development of classification criteria for resistance to soybean rust and differences in virulence among Japanese and Brazilian rust populations. Tropical Plant Pathology 35, 153-162.

Article source : Screening of selected kenyan soybean varieties for resistance to Phakopsora pachyrhizi (Soybean rust) 

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Integrated Management Strategies for Fusarium Root Rot and Wilt in Soybean | InformativeBD

M. Tahmidur Rahman, M. Tanbir Rubayet , Abu Ashraf Khan, and M. Khurshed Alam Bhuiyan,  from the different institute of the Bangladesh. wrote a research article about, Integrated Management Strategies for Fusarium Root Rot and Wilt in Soybean. Entitled, Integrated management of fusarium root rot and wilt disease of soybean caused by Fusarium oxysporum. This research paper published by the International Journal of Biosciences (IJB). an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences | INNSpub. an open access multidisciplinary research journal publisher.

Abstract

An attempt was taken for the management of Fusarium root rot and wilt disease of soybean caused by Fusarium oxysporum using the integration of bio-agent with fungicide and organic amendment. Before going to the field experiments, different in vitro trials were conducted to select a virulent isolate of F. oxysporum, an effective antagonistic isolate of Trichderma harzianum, suitable fungicide and organic amendment. Among the seven isolates of F. oxysporum, FOS-3 isolate was selected as a tested pathogen by the pathogenicity test. On the contrary, among the eighty-seven isolates of T. harzianum, ISR-26 isolate showed the highest (78.70%) inhibition of radial growth of test pathogen. In the case of fungicidal evaluation trial, Provax 200WP was found the most effective fungicide at the lowest conc. (75 ppm) for inhibiting the radial growth of F. oxysporum isolate FOS-3. Additionally, in vitro trial of different organic amendments, mustard oil cake was found the most effective organic amendment for reducing the growth and development of test pathogen at 3% concentration level. In the field trial, integrated use of T. harzianum with Provax 200WP and mustard oil cake under the treatment T9 has appeared the best treatment in reducing seedling mortality (77.67%), disease incidence (81.88%) as well as disease severity (87.51%) caused by the test pathogen. Moreover, treatment T9 was not only the best treatment for the management of soybean disease but also increased the significant quantity of yield (2.25 tha-1). 

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Introduction

Soybean (Glycine max L.) is one of the most important oilseeds crop all over the world. Nowadays, it’s becoming a popular winter crop in Bangladesh. In 2017, the total soybean production in Bangladesh was about 97000 tons (Anon, 2018). There are several factors attributed to the low production of soybean such as climatic conditions, differences in rainfall patterns, an outbreak of diseases and pests etc. Among these factors, plant diseases play a major role in the yield reduction of soybean. More than hundreds of pathogens are known to affect soybean where sixty-six fungi, six bacteria, eight viruses and seven nematodes (Sinclair, 1978).

F. oxysporum is one of the most destructive seedborne as well as soil-borne fungus which can cause root rot and wilt disease of soybean. When F. oxysporum attacks the soybean plant, the lower taproot and lateral roots become brown to black and show cortical decay or prominent vascular discoloration. Finally, the lateral roots may also die and decompose. In the meantime, secondary roots may develop on the upper taproot of the plant. If root rot becomes severe, infected soybeans may develop foliar symptoms including marginal or whole leaf chlorosis, stunting, wilting and finally leaves defoliation. However, the management of this pathogen is difficult because of its long persistence in soil and wide host range. Some chemical fungicides are effective against this fungus but these chemicals are expensive and harmful for living things as well as the environment (Abdel-Monaim et al., 2011). The green revolution has led to intensified agriculture to meet the ever-increasing demands for food and fiber, which is practiced at great cost to the environment, resulting in continuous damage of natural ecosystems, groundwater and food-stuff pollution and other environmental degradation. Indiscriminate use of chemical pesticides and fertilizers in modern agriculture has resulted in the development of several problems such as pesticide resistance in pests, the resurgence of target and non-target pests, destruction of beneficial organisms like honey bees, and chemical residues in food, feed and fodder. However, a few studies have been done on the management of fusarium root rot and wilt disease of soybean but there is no report on integrated management of the above-mentioned disease of soybean in Bangladesh. Considering the aforesaid facts, the present research was undertaken to evaluate the effectiveness of integrated disease management strategies consisted of bio-agent, fungicide and organic amendment against Fusarium root rot and wilt disease of soybean caused by F. oxysporum.

Reference

Abd-El-Khair, Khalifa H, Karima RKM, Haggag HE. 2010. Effect of Trichoderma species on damping off diseases incidence, some plant enzymes activity and nutritional status of bean plants. Journal of American Science 6(9), 486-497.

Abdel-Monaim MF, Ismail ME, Morsy KM. 2011. Induction of systemic resistance in soybean plants against Fusarium wilt disease by seed treatment with benzothiadiazole and humic acid. African Journal of Agricultural Research 6(26), 5747-5756.

Ahmed MU, Bhuiyan MKA, Hossain MM, Rubayet MT, Khaliq QA. 2019. Efficacy of chitosan and bio-agent in controlling southern blight disease of carrot caused by Sclerotium rolfsii and improvement the crop production. Research in: Agricultural & Veterinary Sciences 3(3), 113-125.

Altomare C, Norvell WA, Bjorkmanand T, Harman GC. 1999. Solubilization of phosphates and micronutrients by the plant-growth promoting and biocontrol fungus Trichoderma harzianum Rifai. Applied and Environmental Microbiology 65, 2926-2933.

Anis M, Zaki MJ, Dawar S. 2010. Effect of oilseed cakes alone or in combination with Trichoderma species for the control of charcoal rot of sunflower ( Helianthus annus ). Pakistan Journal of Botany 42(6), 4329 4333.

Anonymous. 2018. Statistical Year Book of Bangladesh. Bangladesh Statistics Division, Ministry of Planning, Govt. of the People’s Republic of Bangladesh, p 318-319.

Arefin MN, Bhuiyan MKA, Rubayet MT. 2019. Integrated use of fungicide, plant extract and bio-agent for management of Alternaria blight disease of Radish (Raphanus sativus L.) and quality seed production. Research in: Agricultural & Veterinary Sciences 3(1), 10-21.

Barnett HL, Hunter BB. 1972. Illustrated genera of imperfect fungi. New York, USA: American Phytopathol. Society Press, p 218.

Begum JA, Bhuiyan KA. 2004. Effect of temperature and pH on Trichoderma harzianum and its sensitivity to fungicides. Bangladesh Journal of Plant Pathology 20, 21-26.

Bhuiyan KA, Sen BC. 2013. Integrated management of southern blight disease of carrot caused by Sclerotium rolfsii. Acta Phytopathologica Sinica 43, 515-523.

Chavan RA, Dhoke PK, Bharose AA, Jadhav VT. 2003. In vitro efficacy of fungicides in controlling Fusarium oxysporum f. sp. carthami. Journal of Soils and Crops 13(1), 98-100.

Das IR, Bhuiyan MKA, Jannat R, Kayesh E, Rubayet MT, Arefin MN. 2019. Effect of bio-fortified compost in controlling soil-borne diseases of lentil (Lens culinaris L.) and enhance the crop growth and yield. Advances in Biology & Earth Sciences 4(2), 93-106.

Dhingra OD, Sinclair JB. 1985. Basic plant pathology methods. CRC Press. Inc. Boca Raton Florida. p 13-44.

Gomez KA, Gomez AA. 1984. Statistical Procedures for Agricultural Research. 2nd ed. NY, USA: John, Wiley and Sons.

Leslie JF, Summerell BA. 2006. The Fusarium Laboratory Manual,” Blackwell Publishing, Oxford, UK. P 388.

Liton MJA, Bhuiyan MKA, Jannat R, Ahmed JU, Rahman MT, Rubayet MT. 2019. Efficacy of Trichoderma-fortified compost in controlling soil-borne diseases of bush bean (Phaseolus vulgaris L.) and sustainable crop production. Advances in Agricultural Science 7(2), 23-136.

Maniruzzaman MK. 2004. Stem canker and Black scraf diseases of potato in Bangladesh: its severity, characteristics of the casual organisum and management practices. Ph.D thesis paper, Department of Botany, Jahangirnagar University, Dhaka, Bangladesh.

Mclean KL, Hunt J, Stewart A. 2001. Compatibility of the biocontrol agent Trichoderma harzianum C52 with selected fungicides. New Zealand Plant Protection 54, 84-88.

Mian IH. 1995. Methods in plant pathology. IPSA-JICA project publication number twenty-four. Institute of Post Graduate Studies in Agriculture, Bangladesh. p 51-52.

Prodhan MSUR. 2007. Integrated control of seedling mortality of tomato caused by Fusarium oxysporum f. sp. lycopercisi. MS thesis paper, Department of Plant Pathology, BSMRAU, Gazipur-1706. Bangladesh.

Rahman MM, Ali MA, Ahmad MU, Dey TK. 2013. Effect of tuber-borne inoculum of Rhizoctonia solani on the development of stem canker and black scurf of potato. Bangladesh Journal of Plant Pathology 29, 29-32.

Rahman MT, Bhuiyan MKA, Karim MA, Rubayet MT. 2018. Screening of soybean resistance genotypes against Fusarium oxysporum, Macrophomina phaseolina, Rhizoctonia solani and Sclerotium rolfsii. Research in: Agricultural & Veterinary Sciences 2(3), 139-156.

Rahman MT. 2006. Investigation on blossom blight (Fusarium equisetti) disease of tube rose and their integrated management. MS thesis, Department of plant pathology, BSMRAU, Gazipur-1706, Bangladesh.

Razaq M, Rab A, Alam H, Salahuddin S, Saud, Ahmad Z. 2015. Effect of potash levels and plant density on potato yield. Journal of Biology, Agriculture and Healthcare 5, 55-62.

Rubayet MT, Bhuiyan MKA, Akanda MAM. 2011. Effect of fungicides, organic amendments and antagonist on in vitro growth of Sclerotium rolfsii. Bangladesh Journal of Plant Pathology 27(1-2), 47-52.

Rubayet MT, Bhuiyan MKA, Jannat R, Masum MMI, Hossain MM. 2018. Effect of biofumigation and soil solarization on stem canker and black scurf diseases of potato (Solanum tuberosum L.) caused by Rhizoctonia solani isolate PR2. Advances in Agricultural Science 6(3), 33-48.

Rubayet MT, Bhuiyan MKA. 2016. Integrated management of stem rot of potato caused by Sclerotium rolfsii. Bangladesh Journal of Plant Pathology 32(1-2), 7-14.

Rubayet MT, Bhuiyan MKA. Hossain MM. 2017. Effect of soil solarization and biofumigation on stem rot disease of potato caused by Sclerotium rolfsii. Annals of Bangladesh Agriculture 21(1-2), 49-59.

Rubayet T, Bhuiyan MKA. 2012. Compatibility of an Isolate of Trichoderma harzianum with fungicides and organic amendments. Bangladesh Journal of Plant Pathology 28(1&2), 63-66.

Sarkar S, Narayanan P, Divakaran A. 2008. The in vitro effect of certain fungicides, insecticides, and biopesticides on mycelial growth in the biocontrol fungus Trichoderma harzianum. Turkish Journal of Biology 34(2010), 399-403.

Simi SA, Jannat R, Rubayet MT, Bhuiyan MKA. 2019. Efficacy of bio-fortified compost in controlling anthracnose disease of chilli caused by Colletotrichum capsici and improvement the crop production. Scholars Academic Journal of Biosciences 7(12), 482-489.

Sinclair JB. 1978. The seed borne nature of some soybean pathogens. The effect of phomopests and Bacillus subtilis on germination and their occurrence in soybean produced in Illinois. Seed Science and Technology (6), 957-964.

Singh DK, Jha MM. 2003. Effect of fungicidal treatment against chickpea wilt caused by Fusarium oxysporum f. sp. ciceri. Journal of Applied Biology & Biotechnology 13(1-2), 41-45.

Sundar AR, Das ND, Krishnaveni D. 1995. In-vitro antagonism of Trichoderma spp. against two fungal pathogens of castor. Indian Journal of Plant Protection 23(2), 152-155.

Yuen GY, Craig MI, Geisler LJ. 1994. Biological control of Rhizoctonia solani on tall fescue using fungal antagonists. Plant Disease 78, 118-123.

Source : Integrated management of fusarium root rot and wilt disease of soybean caused by Fusarium oxysporum

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