Low-Cost Cultivation Protocol for Ganoderma lucidum | InformativeBD

E. Soriano, Veronica P. Mangune, and Melody S. Pascua, from the different institute of the Philippines. wrote a research article about, Low-Cost Cultivation Protocol for Ganoderma lucidum. entitled, Development of low-cost cultivation protocol for Ganoderma lucidum (Curtis) P. Karst. This research paper published by the International journal of Microbiology and Mycology (IJMM). an open access scholarly research journal on Microbiology. under the affiliation of the International Network For Natural Sciences | NNSpub. an open access multidisciplinary research journal publisher.

Abstract

Ganoderma lucidum commonly known as lingzhi mushroom, or reishi mushroom in some countries, is an edible mushroom known for its medicinal value. This study evaluated the optimum culture media, grain spawn and substrate formulation for the cultivation of G. lucidum. The use of different low-cost culture media, grains and substrate formulations in the preparation of pure cultures, grain spawn bags and fruiting bags of G. lucidum were tested. The largest mycelial diameter was observed in Potato Sucrose Agar (93.45mm) which was significantly higher among all the treatments used. It has very thick mycelial density. Cracked corn as spawning material had the shortest incubation period of 14 days, which showed significant difference compared to sorghum seeds and barley grains. The use of cracked corn also incurred the lowest cost and highest return of investment in grain spawn bag production. For fruiting bag production, substrate combination of 50% sawdust and 30% rice straw supplemented with 20% rice bran was the best formulation for fruiting bag production of G. lucidum which had the highest yield with a mean value of 91.30g and biological efficiency of 20.29%.

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Introduction

Mushrooms are considered ultimate healthy food and dietary supplements. They contain proteins, carbohydrates, minerals, vitamins, saturated fatty acids, phenolic compounds, tocopherols, ascorbic acid and carotenoids (Ho et al., 2020). Thus, mushrooms can be used directly in the diet and promote health, taking advantage of both the additive and synergistic effects of all bioactive compounds present in it (Reis et al., 2011).

The cultivation of edible mushrooms could become a way to augment farm income while making use of crop-based residues. The growth of a variety of mushrooms requires different type of substrates and availability of different type of materials. Substrates such as logs, wood sawdust, rice straw and hull, banana leaves, maize stalk, and various grasses can all support mushroom growth (Philippoussis, 2009). In some parts of the Philippines, these substrates may not be available or are available at relatively high prices. Thus, mushroom growers are continuously searching for alternative substrates that may be more readily available or cost effective, or that may provide higher yield and better mushroom quality (Royse D et al., 2004).

Ganoderma lucidum (Curtis) P. Karst is a tropical, edible mushroom that is commonly known as “Reishi” in Japanese and “Lingzhi” in Chinese (Yang and Liau, 1998; Wagner et al., 2003). World-wide, Lingzhi occupies a major source of medicine that has been used for more than 2000 years (Azizi et al., 2012). Commercial G. lucidum products are available in various forms, such as powders, dietary supplements, and tea which are obtained from different parts of the mushroom, including mycelia, spores, and fruiting body (Wachtel-Galor et al., 2011). To meet the gradually increasing demand for G. lucidum as a natural medicine, commercial cultivation of this mushroom has been initiated worldwide, especially in the tropical Asian countries (Chang and Buswell, 2008). As different members of the Ganoderma genus seek different conditions for growth and cultivation, and the traditional cultivation technique takes several months for fruiting body development, artificial cultivation of G. lucidum has been implemented using the available substrates such as grain, sawdust, wood logs and cork residues (Boh et al., 2007). Several substrates have been investigated worldwide for the cultivation of G. lucidum (Tiwari et al., 2004).

With the need to cultivate G. lucidum using lowcost inputs and locally available materials, this project envisioned to determine the best culture media, grain and substrate in terms of biological and cost efficiency. Discovering the best culture media, grain and substrate for G. lucidum may lead to the development of mushroom production technologies that may increase yield, and in effect further increase farmers’ income.

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