Floral Flavonoids: Antioxidant and Antimicrobial Power of Tamarix africana | InformativeBD

Abdelhamid Khabtane,  Azzeddine Zeraib,  Laiche Aouidane,  Wahiba Kara Ali,  Fatima Zohra Belguidoum, and  Chabane Rahmoune, from the different institute of Algeria, wrote a Research article about, Floral Flavonoids: Antioxidant and Antimicrobial Power of Tamarix Africana. Entitled, In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

The aim of this work is to determine the quantity, the quality, the antimicrobial activity and the antioxidant power of various extracts of the flavonoids obtained from the flowers of Tamarix africana Poir. The quantification of the extracts obtained was revealed in high yield of the flavonoids with respectively: the methanoic extracts (26.31%), the extracts of the aqueous phase (19.29%), the extracts of ethyl acetate (0.87%), the extracts of petroleum ether (0.18%). The qualitative study, using the thin-layer chromatography (TLC), showed the dominance of Flavonols, flavones, isoflavones, flavanones and 3-glycosidic Anthocyanidins. The study of microbial activity revealed an important bactericidal power for the extracts of the aqueous phase on Gram + bacteria with a disc of inhibition of 24±1mm on Staphylococcus aureus ATCC43300 and 20±1mm on Staphylococcus aureus ATCC 25923, For the antifungal activity all the extracts gave important effects on Podosphaera leucotrichia (apple powdery mildew), with a maximum disc inhibition of 20±1mm for the ethyl acetate extracts, on the other hand alone The ether extracts of the petrol which showed an inhibitory effect on Penicillium sp. The antioxidant study, expressed as a percentage of DPPH, showed a high efficiency of the various extracts; In particular that of the ethyl acetate extract which inhibits oxidation and traps the free radicals at 100%, which demonstrating the use of this plant in traditional medicine for the treatment of certain types of cancer.

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Introduction

The resistance developed by pathogenic organisms to antibiotics, the spread of many carcinogenic diseases and the excessive use of pesticides polluting ecosystems are reasons that have pushed research towards the exploitation of medicinal plants used Since antiquity using the healing power of their secondary metabolites such as flavonoids, alkaloids, terpenes, etc. (Benabdallah, 2016).

Among these plants are the Tamarix species from the Tamaricaceae family, of which Algeria has more than 15 species of this genus (Khabtane and Rahmoune, 2012). View the use of these plants in traditional medicine in some cases of cancer, diarrhea, hair loss, etc. (Khabtane and Rahmoune, 2010); many studies are carried out on biological activity (antibacterial effect only), neglecting the fungicidal effect, as well as the antioxidant power of the different parts of the Tamarix species such as (Ksouri 2009, Wang, 2009 Saıdana, 2008, Parmar et al., 1994 and ...)

On this vision, our work aims at: the quantitative and qualitative determination of the various extracts of the flavonoids obtained from the floral part of Tamarix africana Poir. The determination of the bactericidal effect and the fungicidal effect which is applied for the first in this work against a fungal species known for its detrimental effects on the production of apple trees (Podosphaera leucotrichia) and finally to put the accent on the antioxidative power of the extracts obtained.

To assess the antimicrobial activity we chosed five species of pathogenic bacteria that are: Staphylococcus aureus ATCC 43300, Escherichia coli, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa, Salmonella sp. for the Fungi We chosed two species: Podosphaera leucotrichia (powdery mildew of apple) which constitutes a threat to the to the arboriculture of apple tree which characterizes the region Khenchela and Penicillium sp.

At the end of the in vitro determination of the antioxidant power we applied the method of Blois (Ben Mansour, 2015), (Biswas 2014), where the free radical DPPH unstable has a dark violet coloration, when it is reduced and the coloration becomes pale yellow

Reference 

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Khabtane A, Rahmoune C. 2010. Contribution à de l’étude de l’effet du biotope sur la richesse floristique et variabilité morphologique chez le Tamarix sp. dans les zones aride de la région de khenchela, thèse du Magister, Université de Constantine, Algérie181-190.

Khabtane A, Rahmoune C. 2012. Effet du biotope sur la diversité floristique et le polymorphisme phénotypique des groupements à Tamarix africana Poir. dans les zones arides de la région de Khenchele (Est Algérien), Journal of Agriculture and Environnent for International Development 106, 123-137.

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Article source :  In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir

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CuO Nanoparticles from Allium Cepa Extract: Green Synthesis and Applications | InformativeBD

Chellappa Karthika, Amutha Eswaran, S Rajaduraipandian, S. andhimathi, and Gurusamy Annadurai,   from the different institute of the India. wrote a research article about, CuO Nanoparticles from Allium Cepa Extract: Green Synthesis and Applications. entitled, Green synthesis of CuO nanoparticles via Allium Cepa extract and its characterizations on dye degradation and antimicrobial activity. This research paper published by the Journal of Biodiversity and Environmental Sciences (JBES). an open access scholarly research journal on Biodiversity. under the affiliation of the International Network For Natural Sciences | NNSpub. an open access multidisciplinary research journal publisher.

Abstract

Industries use dyes in order to colour their products. The discharge of dye bearing wastewater into natural streams, rivers from textile, leather industries make severe environmental problem of the present day. The Heterogeneous photocatalytic process is a high efficiency technique, which is used to oxidize the organic pollutants present in aqueous system. This report aimed to elucidate the Photocatalytic degradation of Malachite green dye in aqueous medium in presence of UV light irradiation using Allium cepa mediated (CuO) Nanoparticle prepared by Bio-method. The as-synthesized copper Nanoparticle was characterized with various techniques UV, FT-IR, XRD, SEM and DLS which reveals many possible interactions of Dye-Nanoparticle system. The maximum photo degradation was obtained at 120min of irradiation time due to their higher surface sites and surface defects. Although the photostablity of Nano copper material (CuO) was also assessed for 4 cycles which ascertained the potential photo catalytic activity and it also proves excellent microbial activity against E-coli and Bacillus. Thus, the Allium cepa mediated Nano copper could play a vital role in environmental remediation of polluted water.

 

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Introduction

The startling technological development has increased the large-scale industrial production of much of the daily-used products with a broad range of applications (Hamza et al., 2018; Meaty et al., 2020; Amr Fouda et al., 2020). Among these products, dyes are crucial in the textile and fashion industries. However, with intensive applications, dyes transform into dangerous pollutants when they are improperly handled and disposed of causing serious environmental and public health hazards (Yoga Lakshmi et al., 2020; Amr Fouda et al., 2020). The increased usage of nanoparticles especially for medical purposes created a new challenge of synthesizing them by untraditional methods to overcome the undeniable disadvantages of other physical and chemical methods (Murali Sastry et al., 2003; Amr Fouda et al., 2020). Plant mediated nanoparticles synthesis (green method) is preferred as it is clean, cost effective, nontoxic and safe for human therapeutic use (Renata Dobrucka and Jolanta Długaszewska, 2015). Onion (Allium cepa) is a widely cultivated plant all over the world. It is rich in carbohydrates, proteins, sodium, potassium and phosphorus. It has been reported to have numerous important properties such as antimicrobial, antioxidant, antiparasitic and anti-inflammatory activities (Johanna, 1999; Eman, 2017; Philip and Unni, 2011). Recently, it is found that onion can be used as a potential candidate for synthesis of CuO nanoparticles. CuO nanoparticles as a narrow band gap semiconductor was investigated extensively due to its promising applications towards sensors, superconductors, solar cells, catalysis, lithium-ion battery and super capacitors, (Zhang et al.,2014). In addition, US Environmental protection agency recognized copper based materials as antimicrobial materials (Dollwet and Sorenson, 2001). The use of CuO nanomaterial in prevention of bacterial infection in medical devices and enhancement of antimicrobial activity towards pathogenic microorganisms in nano form displays is also recorded.

CuO nanomaterials were applied in fabrics, agriculture, paints and also in hospitals due to its desirable properties (Amr Fouda et al., 2020; Kumar et al., 2012). Due to its low cost and toxicity, good chemical and thermal stability with high surface area, CuO based nanomaterials have received considerable attention. In particular, semiconductor nanostructured-based photo catalysis displayed high potential for degradation of various organic pollutants including dyes, surfactants, solvents, pesticides, phenolic compounds, etc. into harmless products under light irradiation. Among nanostructured oxides, copper (II) oxide (CuO) is classified as a semiconductor material type-p with a narrow band gap energy of 1.2 eV and possessing excellent optical, electrical, magnetic, catalytic, and biological properties. In this study, we report the synthesis of CuO nanoparticle by green syn- thesis process using Allium cepa extract as reducing agent. The final products characteristics were studied - SEM, XRD, UV, DLS and FTRI analysis. Along with these characteristics Photocatalytic degradation the antibacterial ability against E. coli and S. Bacillus was examined and reported.

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You-Kang Phang, Mohammod Aminuzzaman, Akhtaruzzaman, Ghulam Muhammad, Sayaka Ogawa, Akira Watanabe, Lai-Hock Tey. 2021. Green Synthesis and Characterization of CuO Nanoparticles Derived from Papaya Peel Extract for the Photocatalytic Degradation of Palm Oil Mill Effluent (POME). Sustainability 13, 796-801.

Source : Green synthesis of CuOnanoparticles via Allium Cepa extract and its characterizations on dyedegradation and antimicrobial activity

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