Showing posts with label Antioxidant activity. Show all posts
Showing posts with label Antioxidant activity. Show all posts

Phytochemical & Antioxidant Profiles of Local and HYV Rice in Bangladesh | InformativeBD

Screening of phytochemical compounds and antioxidant properties in local and HYV of Bangladeshi Rice (Oryza sativa L.)

Mohammad Abdul Mannan, Tushar Chandra Sarker, Md. Mostafizur Rahman, and Mohammad Firoz Alam,  from the institute of Bangladesh. wrote a Research article about, Phytochemical & Antioxidant Profiles of Local and HYV Rice in Bangladesh. Entitled, Screening of phytochemical compounds and antioxidant properties in local and HYV of Bangladeshi Rice (Oryza sativa L.). This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Naturally occurring antioxidant supplements from plants are vital to counter the oxidative damage in cells where consumption of whole grain plays a vital role. As a dietary supplement, antioxidant activities of five local and HYV rice (Kalijira, Chinigura, Hizoldigha, BRRI dhan28, BRRI dhan29) of Bangladesh were examined through DPPH antioxidant assay. Methanol extract of bran, polished and unpolished grain of each genotype were used as a studied sample. Studied sample showed significant antioxidant activity. Where bran is more potent part of rice showed higher antioxidant properties compeering unpolished and polished grain. Unpolished grain also showed greatest result where polished grain showed less performance. Among different genotypes Kalijira bran is black in color and showed better scavenging activity with the IC50 value of 60.12 μg/ml. Hizoldigha unpolished grain is red in color and showed higher antioxidant properties (130.2 μg/ml) compeering other unpolished grain. IC50 value of the positive control as BHT was 37.35 μg/ml. The result of present investigation denotes that the studied genotypes possess moderate antioxidant activity where Kalijira bran bear high antioxidant compound and keep demand to more processing and recently is using for extracting edible oil commonly called as rice bran oil. Unconventional Hizoldigha grain also contain high antioxidant activity and can be considered as nutraceutical foods as staple food.

Submit your article to IJB Journal

Read more : Growth, Sex Ratio & Fruit Yield of Juniperus excelsa in Mastuj Valley | InformativeBD 

Introduction

It is widely recognized that dietary ingredients have a dual role, one of them is nutritional and another is pharmaceuticals. So now it’s often called nutracuticals. In recent years, cereals and its ingredients are accepted as functional foods and nutraceuticals because of providing dietary fiber, proteins, energy, minerals, vitamins and antioxidants required for human health. Plant derived antioxidant such as ascorbic acid, tocopherols, carotenoids and phenolic compounds (polyphenols) (Choi et al., 2007), besides other bioactive compounds are reported to have antioxidants activity. Currently, synthetic antioxidants such as butylated hydroxytoluene (BHT) butylated hydroxyanisole (BHA), propyl gallate (PG) and tert-butylhydroquinone (TBHQ) are used under strict regulations because of their toxic effects on human enzyme systems (Hatate et al., 1990, Hattori et al., 1998). In contrast, natural antioxidants have attracted more and more interests because of their safety and wide distribution properties (Lewis, 1993).

The phytochemicals in fruits and vegetables are different from those in the grains, which contain tocotrienols and tocopherol, while rice is contain oryzanol (Lloyd et al., 2000). The phenolic like ferulic acid and diferulate are predominant in grains, but are not significant in some fruit and vegetables (Bunzel et al., 2001). Thus, the regular insertion of cereals and their processed products can make a payment to health endorsement and disease avoidance (Chaturvedi et al., 2011).

Rice, being one of the most produced and consumed cereals in the world (FAO, 1995), has an important role in the relation between the diet and health. Several compounds with antioxidant activity have been identified in rice, including phenolic compounds, tocopherols, tocotrienols and γ-oryzanol (Iqbal et al., 2005). Among them phenolic compounds is one of most important that are secondary metabolites of plants, with different activities such as protection against pathogens and predators, mechanical support, attraction of pollinating animals, and protection against ultraviolet radiation (Parr and Bolwell, 2000). Several phenolic compounds have already been identified in rice. The phenolic compounds are mainly associated with the pericarp in rice; hence, the milling process reduces the concentration of these compounds in the grain. Besides, grains with darker pericarp colour, such as red and black rice, contain higher amounts of polyphenols (Tian et al., 2004). The concentration of total phenolics in the grain has been positively associated with the antioxidant activity (Zhang et al., 2006).

Rice bran is an underutilized co-product from rice milling and generally used as animal feed, although it has long been considered an excellent source of vitamins and other nutrients. Bidlack (1999) has shown that rice bran may contain over 100 different antioxidants. Lloyd et al. (2000) also reported that, rice bran contains high amounts of beneficial antioxidants including tocopherols, tocotrienols, and oryzanols. It is also remarkable that, antioxidants containing level also depend on the type of rice (Gaydou et al., 1980). However if we see the rank of antioxidant rich food, than it will be clearer that the color fruits, vegetables, spices and nuts are more potent to show antioxidant activity than grain. But all of those are expansible and not edible as much as we need where rice is only foods that we take maximum amount per day and suitable for all classes of people. So if we could find out the high antioxidant compound containing rice genotypes and increase the amount of those phytochemicals in our daily diet rice, than it would be also beneficial like golden rice. Studied genotypes Kalijira and Chinigura are local aromatic varieties and small in size, Hizoldigha is low yielding local Amon varieties with red color pericarp and normally grown in deep water where BRRI dhan28 and BRRI dhan29 are modern transplanted high yielding varieties of Bangladesh.

The present investigation was designed to evaluate the phytochemical screening and antioxidant activity of rice genotypes generally cultivated if Bangladesh and are important in different aspects. Here DPPH antioxidant assay was used to evaluate the antioxidant activity of selected sample because scavenging of DPPH radical is the basis of the popular DPPH antioxidant assay (Kordali et al., 2005).

Reference

Abbas A, Murtaza S, Aslam F, Khawar A, Rafique S, Naheed S. 2011. Effect of processing on nutritional value of rice (Oryza sativa L.). World Journal of Medical Science 6(2), 68-73.

Adom KK, Liu RH. 2002. Antioxidant activity of grains. Journal of Agricultural and Food Chemistry 50, 6182-6187. http://dx.doi.org/10.1021/jf0205099

Ahmad I, Beg Z. 2001. Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens. Journal of Ethnopharmacology 74, 87-91. http://dx.doi.org/10.1016/S0378-8741(00)00335-4

Akueshi CO, Kadiri CO, Akueshi EU, Agina SE, Ngurukwem B. 2002. Antimicrobial potentials of Hyptis sauvedens Poit (Lamiaccae). Nigeria Journal of Botany 15, 37-41.

Bidlack W. 1999. Phytochemicals as bioactive agents, Technomic Publishing Co. Inc., Lancaster, Basel, Switzerland, p. 25-36.

Bunzel M, Ralph J, Martia JM, Hatfield Rd, Steinhart H. 2001. Diferulates as structural components in soluble and insoluble cereal dietary fiber. Journal of the Science of Food and Agriculture 81, 653-660.

Chatha SAS, Anwar F, Manzoor M, Bajwa J. 2006. Evaluation of the antioxidant activity of rice bran extracts using different antioxidant assays. Grasas y aceites 57(3), 328-335.

Chaturvedi N, Sharma P, Shukla K, Singh R, Yadav S. 2011. Cereals Nutraceuticals, Health Ennoblement and Diseases Obviation: A Comprehensive Review. Journal of Applied Pharmaceutical Science 01(7), 06-12.

Choi HY, Jhun EJ, Lim BO. 2000. Application of flow injection-chemilumineacence to the study of radical scavenging activity in plant. Phytotherapy 14, 250-253.

Choi Y, Jeong HS, Lee J. 2007. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chemistry 103, 130-138. http://dx.doi.org/10.1016/j.foodchem.2006.08.004

Chotimarkorn C, Benjakul S, Silalai N. 2008. Antioxidant components and properties of five long-grained rice bran extracts from commercial available cultivars in Thailand. Food Chemistry 111, 636–641. http://dx.doi.org/10.1016/j.foodchem.2008.04.031

Ekwenye UN, Elegalam NN. 2005. Antibacterial activity of Ginger (Zingiber officinale Roscoe and Garlic (Allium sativum L.) extracts on Escherichia coli and Salmonella typhi. International Journal of Molecular and Advance Science 1(4), 411-416.

FAO. 1995. Food and Agriculture Organization. Land resource appraisal of Bangladesh for agricultural development, 17pp.

Gaydou EM, Raonizafinimanana R, Bianchini JP. 1980. Quantitative analysis of fatty acids and sterols in Malagasy rice bran oils. Journal of the American Oil Chemists’ Society 57, 141-142.

Harbone JB. 1973. Phytochemical methods, London. Chapman and Hall, ltd.pp.49-188.

Hatate H, Nagata Y, Kochi M. 1990. Antioxidant effect of bovine serum albumin hydrolyzates and their synergistics effect with antioxidants. Yukagaku 39, 42–46.

Hattori M, Yamaji TK, Kumagai H, Feng Y, Takahashi K. 1998. Antioxidative peptides from food proteins A review. Journal of Agricultural and Food Chemistry 46, 2167–2170.

Iqbal S, Bhanger MI, Anwar F. 2005. Antioxidant properties and components of some commercially available varieties of rice bran in Pakistan. Food Chemistry 93, 265-272.

Kong JM, Chia LS, Goh NK, Chia TF, Brouuillard R. 2003. Analysis and biological activities of anthocyanins. Phytochemistry 64, 923-933.http://dx.doi.org/10.1016/S0031-9422(03)00438-2

Kordali S, Cakir A, Mavi A, Kilic H, Yildirim A. 2005. Screening of chemical composition and antifungal and antioxidant activities of the essential oils from three Turkish Artemisia species. Journal of Agricultural and Food Chemistry 53, 1408–1416.

Laokuldilok T, Charles F, Shoemaker, Jongkaewwattana S, Tulyathan V. 2011. Antioxidants and Antioxidant Activity of Several Pigmented Rice Brans. Journal of Agricultural and Food Chemistry 59, 193–199.

Lewis NG. 1993. Plant phenolics. In: Alscher RG, Hess JL (eds) Antioxidants in higher plants. Boca Raton, FL, CRC Press, pp. 135–160.

Lloyd BJ, Siebenmorgen TJ, Beers KW. 2000. Effects of commercial processing on antioxidants in rice bran. Cereal Chemistry 77(5), 551–555. http://dx.doi.org/10.1094/CCHEM.2000.77.5.551

Min B, Gu L, Anna M, McClung, Christine J, Bergman, Chen MH. 2012. Free and bound total phenolic concentrations, antioxidant capacities, and profiles of proanthocyanidins and anthocyanins in whole grain rice (Oryza sativa L.) of different bran colours. Food Chemistry 133, 715–722. http://dx.doi.org/10.1016/j.foodchem.2012.01.079

Nam SH, Choi SP, Kang MY, Koh HJ, Kozukue N, Friedman M. 2006. Antioxidative activities of bran extracts from twenty one pigmented rice cultivars. Food Chemistry 94(4), 613–620.

Parr AJ, Bolwell GP. 2000. Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profile. Journal of the Science of Food and Agriculture 80, 985-1012. http://dx.doi.org/10.1002/(SICI)1097-0010(20000515)80:7<985::AID-JSFA572>3.0.CO;2-7

Rao AS, Sareddy G, Phanithi P, Babu, Reddy AR. 2010. The antioxidant and antiproliferative activities of methanolic extracts from Njavara rice bran. BMC complementary and alternative medicine 34, 109.

Romero MV,  Panajon  NM, Manaoes  RV, Mamucod HF. 2009. Health-promoting antioxidants from pigmented rice. Philippine Journal of Crop Science 34(1), 110.

Rossi A, Serraino I, Dugo P, Paola RD, Mondello L, Genovese T. 2003. Protective effects of anthocyanins from blackberry in a rat model of acute lung inflammation. Free Radical Research 37, 891–900.

Ryu SN, Park SZ, Ho CT. 1998. High performance liquid chromatographic determination of anthocyanin pigments in some varieties of black rice. Journal of Food and Drug Analysis 6, 729–736.

Sofowara A. 1993. Medicinal plants and Traditional medicine if Africa. Spectrum Books Ltd,Ibadan, Nigeria. p. 289.

Srisawat U, Panunto W, Kaendee N, Tanuchit S, Itharat A, Lerdvuthisopon N, Hansakul P. 2010. Determination of phenolic compounds, flavonoids, and antioxidant activities in water extracts of Thai red and white rice cultivars. Journal of the Medical Association of Thailand 93(7), 83-91.

Tian S, Nakamura K, Kayahara H. 2004. Analysis of phenolic compounds in white rice, brown rice, and germinated brown rice. Journal of Agricultural and Food Chemistry 52, 4808-4813.

Trease GE. 1989. Evens EC Pharmacology. 11th edn. Brailliar Tiridel Can. Macmillian publishaer.

Walter M, Marchesan E. 2011. Phenolic compounds and antioxidant activity of rice. Brazilian Archives of Biology and Technology 54(1), 371-377.

Yafang S, Gan, Jinsong B. 2011. Total phenolic content and antioxidant capacity of rice grains with extremely small size. African Journal of Agricultural Research 6(10), 2289-2293.

Yodmanee S, Karrila TT, Pakdeechanuan P. 2011. Physical, chemical and antioxidant properties of pigmented rice grown in Southern Thailand. International Food Research Journal 18(3), 901-906.

Zhang M, Guo B, Zhang R, Chi J, We Z, Xu Z, Zhang Y, Tang X. 2006. Separation, purification and identification of antioxidant compositions in black rice. Agricultural Science in China 5, 431-440.

Tian S, Nakamura K, Kayahara H. 2004. Analysis of phenolic compounds in white rice, brown rice, and germinated brown rice. Journal of Agricultural and Food Chemistry 52, 4808-4813.

Zhou Z, Robards K, Helliwell S, Blanchard C. 2004. The distribution of phenolic acids in rice. Food Chemistry 87, 401-406.

Tian S, Nakamura K, Cui T, Kayahara H. 2005. High-performance liquid chromatographic determination of phenolic compounds in rice. Journal of Chromatography A 1063, 121-128. http://dx.doi.org/10.1016/j.chroma.2004.11.075

Hudson E A, Dinh PA, Kokubun T, Simmonds MSJ, Gescher A. 2000. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. Cancer Epidemiology, Biomarkers & Prevention 9, 1163-1170.

Chen P, Kuo W, Chiang C, Chiou H, Hsieh Y, Chu S. 2006. Black rice anthocyanins inhibit cancer cells invasion via  repressions of MMPs and u-PA expression. Chemico-Biological Interactions 163, 218-229. http://dx.doi.org/10.1016/j.cbi.2006.08.003

Yawadio R, Tanimori S, Morita N. 2007. Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities. Food Chemistry 101, 1616-1625. http://dx.doi.org/10.1016/j.foodchem.2006.04.016

Article source : Screening of phytochemical compounds and antixidant properties in local and HYV of Bangladeshi Rice (Oryza sativa L.) 

 

Floral Flavonoids: Antioxidant and Antimicrobial Power of Tamarix africana | InformativeBD

In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir

Abdelhamid Khabtane,  Azzeddine Zeraib,  Laiche Aouidane,  Wahiba Kara Ali,  Fatima Zohra Belguidoum, and  Chabane Rahmoune, from the different institute of Algeria, wrote a Research article about, Floral Flavonoids: Antioxidant and Antimicrobial Power of Tamarix Africana. Entitled, In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

The aim of this work is to determine the quantity, the quality, the antimicrobial activity and the antioxidant power of various extracts of the flavonoids obtained from the flowers of Tamarix africana Poir. The quantification of the extracts obtained was revealed in high yield of the flavonoids with respectively: the methanoic extracts (26.31%), the extracts of the aqueous phase (19.29%), the extracts of ethyl acetate (0.87%), the extracts of petroleum ether (0.18%). The qualitative study, using the thin-layer chromatography (TLC), showed the dominance of Flavonols, flavones, isoflavones, flavanones and 3-glycosidic Anthocyanidins. The study of microbial activity revealed an important bactericidal power for the extracts of the aqueous phase on Gram + bacteria with a disc of inhibition of 24±1mm on Staphylococcus aureus ATCC43300 and 20±1mm on Staphylococcus aureus ATCC 25923, For the antifungal activity all the extracts gave important effects on Podosphaera leucotrichia (apple powdery mildew), with a maximum disc inhibition of 20±1mm for the ethyl acetate extracts, on the other hand alone The ether extracts of the petrol which showed an inhibitory effect on Penicillium sp. The antioxidant study, expressed as a percentage of DPPH, showed a high efficiency of the various extracts; In particular that of the ethyl acetate extract which inhibits oxidation and traps the free radicals at 100%, which demonstrating the use of this plant in traditional medicine for the treatment of certain types of cancer.

Submit your article to IJB Journal

Read moreTracking Tomato Resistance: Potato Virus Y Infections in Pakistan | InformativeBD 

Introduction

The resistance developed by pathogenic organisms to antibiotics, the spread of many carcinogenic diseases and the excessive use of pesticides polluting ecosystems are reasons that have pushed research towards the exploitation of medicinal plants used Since antiquity using the healing power of their secondary metabolites such as flavonoids, alkaloids, terpenes, etc. (Benabdallah, 2016).

Among these plants are the Tamarix species from the Tamaricaceae family, of which Algeria has more than 15 species of this genus (Khabtane and Rahmoune, 2012). View the use of these plants in traditional medicine in some cases of cancer, diarrhea, hair loss, etc. (Khabtane and Rahmoune, 2010); many studies are carried out on biological activity (antibacterial effect only), neglecting the fungicidal effect, as well as the antioxidant power of the different parts of the Tamarix species such as (Ksouri 2009, Wang, 2009 Saıdana, 2008, Parmar et al., 1994 and ...)

On this vision, our work aims at: the quantitative and qualitative determination of the various extracts of the flavonoids obtained from the floral part of Tamarix africana Poir. The determination of the bactericidal effect and the fungicidal effect which is applied for the first in this work against a fungal species known for its detrimental effects on the production of apple trees (Podosphaera leucotrichia) and finally to put the accent on the antioxidative power of the extracts obtained.

To assess the antimicrobial activity we chosed five species of pathogenic bacteria that are: Staphylococcus aureus ATCC 43300, Escherichia coli, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa, Salmonella sp. for the Fungi We chosed two species: Podosphaera leucotrichia (powdery mildew of apple) which constitutes a threat to the to the arboriculture of apple tree which characterizes the region Khenchela and Penicillium sp.

At the end of the in vitro determination of the antioxidant power we applied the method of Blois (Ben Mansour, 2015), (Biswas 2014), where the free radical DPPH unstable has a dark violet coloration, when it is reduced and the coloration becomes pale yellow

Reference 

Athamena S. 2009. Etude Quantitative Des Flavonoides Des Graines De Cuminum Cyminum Et Les Feuilles De Rosmarinus officinalis Et L’evaluation De L’activite Biologique. Biochimie Appliquée 7, 126-133

Ben Mansour A, Porter EA, Kite GC, Simmonds MS, Abdelhedi R, Bouaziz M. 2015. Phenolic profile characterization of Chemlali olive stones by liquid chromatography-ion trap mass spectrometry. J Agric Food Chem 63, 1990-1995.

Benabdallah A, Rahmoune C, Boumendjel M, Aissi O, Messaoud C. 2016. Total phenolic content and antioxidant activity of six wild Mentha species (Lamiaceae) from Northeast of Algeria. Asian Pac J Trop Biomed 6, 760-766.

Biswas N, Saha S, Ali MK. 2014. Antioxidant, antimicrobial, cytotoxic and analgesic activities of ethanolic extract of Mentha arvensis L. Asian Pac J Trop Biomed 4, 792-798.

Bonina F, Lanza M, Montenegro L, Puglisi C, Tomaino A, Trombetta D, Castelli F, Saija A. 1996. Flavonoids as potential protective agents against photo-oxidative skin damage. International Journal of Pharmaceutics 145, 87-94.

Celiktas OY, Hames Kocabas, EE, Bedir, E, Vardar Sukan F, Ozek T, Baser KHC. 2007. Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chem 100, 553-559.

Huang G, Jiang J, Dai D. 2008. Antioxidative and antibacterial activity of the methanol extract of Artemisia anomala S. Moore. African Journal of Biotechnol. 7, 1335-1338.

Ishtiaq S, Ahmad M, Hanif U, Akbar S, Mehjabeen, Kamran SH. 2014. Phytochemical and in vitro antioxidant evaluation of different fractions of Amaranthus graecizans subsp. silvestris (Vill.) Brenan. Asian Pac J Trop Med 7, 342-347.

Khabtane A, Rahmoune C. 2010. Contribution à de l’étude de l’effet du biotope sur la richesse floristique et variabilité morphologique chez le Tamarix sp. dans les zones aride de la région de khenchela, thèse du Magister, Université de Constantine, Algérie181-190.

Khabtane A, Rahmoune C. 2012. Effet du biotope sur la diversité floristique et le polymorphisme phénotypique des groupements à Tamarix africana Poir. dans les zones arides de la région de Khenchele (Est Algérien), Journal of Agriculture and Environnent for International Development 106, 123-137.

Ksouri R, Falleh H, Megdiche W, Trabelsi N, Mhamdi B, Chaieb K, Bakrouf A, hristian Magné C, Abdelly C. 2009. Antioxidant and antimicrobial activities of the edible medicinal halophyte Tamarix gallica L. and related polyphenolic constituents. Food and Chemical Toxicology 47, 2083-2091.

Marfak A. 2003. Radiolyse gamma des flavonoïdes. Etude de Leur reactivite avec les radicaux issus des Alcools: formation de depsides. Thèse de doctorat, Université Limoges, Belgique 225-230.

Markowicz Bastos DH, Saldanha LA, Catharino RR, Sawaya ACHF, Cunha IBS, Carvalho PO, Eberlin MN. 2007. Phenolic Antioxidants Identified by ESI-MS from Yerba Maté (Ilex paraguariensis) and Green Tea (Camelia sinensis) Extracts. Molecules 12, 423-432.

Parmar VS, Bisht KS, Sharma SK, Jain R, Taneja P, Singh S, Simonsen O, Boll PM. 1994. Highly oxygenated bioactive flavones from Tamarix. Phytochemistry 36, 507-511.

Rasooli I, Fakoor MH, Yadegarinia D, Gachkar L, Allameh A, Rezaei MB. 2008. Antimycotoxigenic characteristics of Rosmarinus officinalis and Trachyspermum copticum L. essential oils. International J of Food Microbiology 122, 135-139.

Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R. 2005. Comparative evaluation of 11 essential oils of different origin as functional antioxidants, antiradicals and antimicrobials in foods. Food Chem 91, 621-632.

Saıdana D, Mahjoub MA, Boussaada O, Chriaa J, Cheraif I, Daami M, Mighri Z, Helal AN. 2008. Chemical composition and antimicrobial activity of volatile compounds of Tamarix boveana (Tamaricaceae). Microbiological Research 163, 445-455.

Wang B, Ren S, Li G, Guan H. 2009. Studies on antitumor steroids and flavonoids from Tamarix chinensis Lour. Chinese Pharmacetical Journal 44, 576-580.

Article source :  In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir

Comparative Antioxidant Activity of Popular Philippine Herbal Teas | InformativeBD

A comparative study on the antioxidant activity of selected Philippine herbal teas

Amanda Villaggi, Victoria Carranza,  Ariana Defrancesco, Judy Kristel V. Bayalas, Rollan Paul Parakikay, Jose Rene L. Micor,  and Elmer-Rico E. Mojica, from the different institute of the United States and  Philippines. wrote a Research Article about, Comparative Antioxidant Activity of Popular Philippine Herbal Teas. Entitled, A comparative study on the antioxidant activity of selected Philippine herbal teas. This research paper published by the International Journal of Biosciences (IJB). an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences | INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Tea ranks as the second most consumed beverage worldwide, surpassed only by water. With an annual production of approximately 2.5 million metric tons of dried tea, its popularity has surged in recent years, largely due to its recognized health benefits, particularly as a rich source of potent antioxidants. This study focused on evaluating the total phenolic content of seven commercial herbal tea samples from the Philippines using the Folin-Ciocalteu method. Additionally, antioxidant activity was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assays, and the findings were correlated to the phenolic content. The results revealed that mango tea showed the highest phenolic content followed by pito-pito tea. In terms of antioxidant efficacy, the pito-pito tea showed the lowest IC50 value in the DPPH assay, while mango had the lowest IC50 value in the ABTS assay, followed closely by pito-pito. A correlation was found between the phenolic content and the antioxidant activities of the teas.

Submit your article to IJB Journal

Introduction

Tea, one of the world’s most popular beverages, holds profound cultural and historical significance across various societies. With over 2 billion cups consumed daily, tea is produced in more than 60 countries, demonstrating its universal appeal. Traditionally, tea is made from the dried leaves of the Camellia sinensis plant, celebrated for its numerous health benefits. Green tea, for instance, is known to boost the immune system and improve cardiovascular health, while black tea has been linked to a reduced risk of chronic diseases such as heart disease and diabetes (Khan and Mukhtar, 2007). However, as awareness of the potential negative effects of caffeine has grown, many people have turned to herbal teas as a caffeine-free alternative that still offers a variety of health benefits.

Unlike traditional tea, herbal teas are not derived from the Camellia sinensis plant. Instead, they are made from a blend of ingredients known as tisanes, which include leaves, bark, nuts, fruits, and other botanical elements. These herbal blends are prized not only for their flavor but also for their medicinal properties. Often crafted with specific health outcomes in mind, herbal teas can help boost the immune system, provide antioxidants, or promote relaxation (Serafini et al., 2011). For example, chamomile tea is well-regarded for its calming effects, while peppermint tea is commonly used to alleviate headaches and improve digestion (McKay and Blumberg, 2006).

The global rise in the popularity of herbal teas has coincided with a growing interest in natural antioxidants, which play a crucial role in maintaining the body’s balance by neutralizing harmful reactive oxygen species (ROS) linked to degenerative diseases (Bocci and Valacchi, 2013). Recent studies have highlighted a preference for natural antioxidants like those found in herbal teas over synthetic ones as these have been associated with toxicity and carcinogenicity (Pokorny, 2007). This trend underscores the increasing demand for healthier, plant-based alternatives in both dietary and medicinal contexts. 

In the Philippines, a country rich in biodiversity, herbal plants have long played a vital role in traditional medicine and are deeply embedded in the cultural fabric of the nation. Although many of these plants are not indigenous to the region, they have been used for centuries for their medicinal properties, contributing to a rich historical legacy. Philippine herbal teas, such as those made from guava, mango, guyabano, pito-pito, malunggay, and ampalaya, have gained popularity for both their health benefits and cultural significance. These teas are typically prepared by brewing or boiling plant materials in water, and are often enhanced with sweeteners or spices to improve their flavor. A growing body of literature emphasizes the importance of understanding the benefits of various Philippine herbal products to ensure their safe and effective use (Maramba-Lazarte, 2020), particularly in light of ongoing research into remedies for diseases like COVID19, diabetes, and hypertension.

This study focuses on six popular Philippine-based herbal teas, examining their potential health benefits by analyzing their polyphenol content and antioxidant activity. The research aims to provide a deeper understanding of how these locally sourced herbal teas contribute to health and well-being, aligning with the global trend of turning to natural, plant-based remedies for maintaining health and preventing disease.

Reference

Awny MM, Al-Mokaddem AK, Ali BM. 2021. Mangiferin mitigates di-(2-ethylhexyl) phthalate-induced testicular injury in rats by modulating oxidative stress-mediated signals, inflammatory cascades, apoptotic pathways, and steroidogenesis. Archives of Biochemistry and Biophysics 711, 108982. DOI: 10.1016/j.abb.2021.108982.

Baskar R, Rajeswari V, Kumar TS. 2007. In vitro antioxidant studies in leaves of Annona species. Indian Journal of Experimental Biology 45, 480-485.

Berardini N, Fezer R, Conrad J, Beifuss U, Carle R, Schieber A. 2005. Screening of mango (Mangifera indica L.) cultivars for their contents of flavonol O- and xanthone C-glycosides, anthocyanins, and pectin. Journal of Agricultural and Food Chemistry 53, 1563-1570.

Bocci V, Valacchi G. 2013. Free radicals and antioxidants: how to reestablish redox homeostasis in chronic diseases? Current Medical Chemistry 20(27), 3397-3415. DOI: 10.2174/0929867311320270005.

Coria A, Montalvo-González E, Yahia E, Obledo-Vázquez E. 2016. Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action, and toxicity. Arabian Journal of Chemistry 11. DOI: 10.1016/j.arabjc.2016.01.004.

Fahey JW. 2005. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees for Life Journal 1, December.

Garrido G, González D, Lemus Y, García D, Lodeiro L, Quintero G, Delporte C, Núñez-Sellés AJ, Delgado R. 2004. In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG). Pharmacological Research 50, 143-149. DOI: 10.1016/j.phrs.2003.12.003.

Jiménez-Escrig A, Rincón M, Pulido R, Saura-Calixto F. 2001. Guava fruit (Psidium guajava L.) as a new source of antioxidant dietary fiber. Journal of Agricultural and Food Chemistry 49, 5489-5493. DOI: 10.1021/jf010147p.

Khan N, Mukhtar H. 2007. Tea polyphenols for health promotion. Life Sciences 81(7), 519-533. DOI: 10.1016/j.lfs.2007.06.011.

Kubola J, Siriamornpun S. 2008. Phenolic contents and antioxidant activities of bitter gourd (Momordica charantia L.) leaf, stem, and fruit fraction extracts in vitro. Food Chemistry 110, 881-890. DOI: 10.1016/j.foodchem.2008.02.076.

Liu FX, Fu SF, Bi XF, Chen F, Liao XJ, Hu XS, Wu JH. 2013. Physico-chemical and antioxidant properties of four mango (Mangifera indica L.) cultivars in China. Food Chemistry 138, 396-405. DOI: 10.1016/j.foodchem.2012.09.111.

Luo Y, Peng B, Wei W, Tian X, Wu Z. 2019. Antioxidant and anti-diabetic activities of polysaccharides from guava leaves. Molecules 24, 1343. DOI: 10.3390/molecules24071343.

Maramba-Lazarte CC. 2020. Benefits of mainstreaming herbal medicine in the Philippine healthcare system. Acta Medica Philippina 54(1). DOI: 10.47895/amp.v54i1.1078.

McKay DL, Blumberg JB. 2006. A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.). Phytotherapy Research 20(7), 519-530. DOI: 10.1002/ptr.1900.

Pokorný J. 2007. Are natural antioxidants better – and safer – than synthetic antioxidants? European Journal of Lipid Science and Technology 109, 629-642. DOI: 10.1002/ejlt.200700064.

Rahmani AH, Almatroudi A, Allemailem KS, Alharbi HOA, Alwanian WM, Alhunayhani BA, Algahtani M, Theyab A, Almansour NM, Algefary AN, Aldeghaim SSA, Khan AA. 2023. Role of mangiferin in the management of cancers through modulation of signal transduction pathways. Biomedicines 11, 3205. DOI: 10.3390/biomedicines11123205.

Ramos JLT, De Castro-Cruz KA, Hsieh C-L, Tsai P-W. 2021. [Title not available]. Plant Cell Biotechnology and Molecular Biology 22, 34-52.

Ruksiriwanich W, Khantham C, Muangsanguan A, Phimolsiripol Y, Barba FJ, Sringarm K, Rachtanapun P, Jantanasakulwong K, Jantrawut P, Chittasupho C, Chutoprapat R, Boonpisuttinant K, Sommano SR. 2022. Guava (Psidium guajava L.) leaf extract as bioactive substances for anti-androgen and antioxidant activities. Plants 11, 3514. DOI: 10.3390/plants11243514.

Serafini M, Del Rio D, Yao DN, Bettuzzi S, Peluso I. 2011. Health benefits of tea. In: Benzie IFF, Wachtel-Galor S, editors. Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. Boca Raton (FL): CRC Press/Taylor and Francis; Chapter 12. Available from: https://www.ncbi.nlm.nih.gov/books/NBK92768/

Siddhuraju P, Becker K. 2003. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of drumstick tree (Moringa oleifera Lam.) leaves. Journal of Agricultural and Food Chemistry 51, 2144-2155. DOI: 10.1021/jf020444+.

Verma AR, Vijayakumar M, Mathela CS, Rao CV. 2009. In vitro and in vivo antioxidant properties of different fractions of Moringa oleifera leaves. Food and Chemical Toxicology 47, 2196-2201. DOI: 10.1016/j.fct.2009.06.005.

Vijayameena C, Subhashini G, Loganayagi M, Ramesh B. 2013. Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. International Journal of Current Microbiology and Applied Sciences 2, 1-8.

Yehia RS, Altwaim SA. 2023. An insight into in vitro antioxidant, antimicrobial, cytotoxic, and apoptosis induction potential of mangiferin, a bioactive compound derived from Mangifera indica. Plants 12, 1539. DOI: 10.3390/plants12071539.

Source A comparative study on the antioxidant activity of selected Philippine herbal teas