Floral Flavonoids: Antioxidant and Antimicrobial Power of Tamarix africana | InformativeBD

Abdelhamid Khabtane,  Azzeddine Zeraib,  Laiche Aouidane,  Wahiba Kara Ali,  Fatima Zohra Belguidoum, and  Chabane Rahmoune, from the different institute of Algeria, wrote a Research article about, Floral Flavonoids: Antioxidant and Antimicrobial Power of Tamarix Africana. Entitled, In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

The aim of this work is to determine the quantity, the quality, the antimicrobial activity and the antioxidant power of various extracts of the flavonoids obtained from the flowers of Tamarix africana Poir. The quantification of the extracts obtained was revealed in high yield of the flavonoids with respectively: the methanoic extracts (26.31%), the extracts of the aqueous phase (19.29%), the extracts of ethyl acetate (0.87%), the extracts of petroleum ether (0.18%). The qualitative study, using the thin-layer chromatography (TLC), showed the dominance of Flavonols, flavones, isoflavones, flavanones and 3-glycosidic Anthocyanidins. The study of microbial activity revealed an important bactericidal power for the extracts of the aqueous phase on Gram + bacteria with a disc of inhibition of 24±1mm on Staphylococcus aureus ATCC43300 and 20±1mm on Staphylococcus aureus ATCC 25923, For the antifungal activity all the extracts gave important effects on Podosphaera leucotrichia (apple powdery mildew), with a maximum disc inhibition of 20±1mm for the ethyl acetate extracts, on the other hand alone The ether extracts of the petrol which showed an inhibitory effect on Penicillium sp. The antioxidant study, expressed as a percentage of DPPH, showed a high efficiency of the various extracts; In particular that of the ethyl acetate extract which inhibits oxidation and traps the free radicals at 100%, which demonstrating the use of this plant in traditional medicine for the treatment of certain types of cancer.

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Introduction

The resistance developed by pathogenic organisms to antibiotics, the spread of many carcinogenic diseases and the excessive use of pesticides polluting ecosystems are reasons that have pushed research towards the exploitation of medicinal plants used Since antiquity using the healing power of their secondary metabolites such as flavonoids, alkaloids, terpenes, etc. (Benabdallah, 2016).

Among these plants are the Tamarix species from the Tamaricaceae family, of which Algeria has more than 15 species of this genus (Khabtane and Rahmoune, 2012). View the use of these plants in traditional medicine in some cases of cancer, diarrhea, hair loss, etc. (Khabtane and Rahmoune, 2010); many studies are carried out on biological activity (antibacterial effect only), neglecting the fungicidal effect, as well as the antioxidant power of the different parts of the Tamarix species such as (Ksouri 2009, Wang, 2009 Saıdana, 2008, Parmar et al., 1994 and ...)

On this vision, our work aims at: the quantitative and qualitative determination of the various extracts of the flavonoids obtained from the floral part of Tamarix africana Poir. The determination of the bactericidal effect and the fungicidal effect which is applied for the first in this work against a fungal species known for its detrimental effects on the production of apple trees (Podosphaera leucotrichia) and finally to put the accent on the antioxidative power of the extracts obtained.

To assess the antimicrobial activity we chosed five species of pathogenic bacteria that are: Staphylococcus aureus ATCC 43300, Escherichia coli, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa, Salmonella sp. for the Fungi We chosed two species: Podosphaera leucotrichia (powdery mildew of apple) which constitutes a threat to the to the arboriculture of apple tree which characterizes the region Khenchela and Penicillium sp.

At the end of the in vitro determination of the antioxidant power we applied the method of Blois (Ben Mansour, 2015), (Biswas 2014), where the free radical DPPH unstable has a dark violet coloration, when it is reduced and the coloration becomes pale yellow

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Article source :  In vitro evaluation of the anti-microbial activity and the anti-oxidant activity of the flavonoids extracted from the flowers of the Tamarix africana Poir