Isolation and Identification of Bacterial Pathogens in Infected Shing Fish (Heteropneustes fossilis) from Freshwater Ponds in Bangladesh | InformativeBD

Mohammad Zakerin Abedin, from the institute of Bangladesh. Rubait Hasan, from the institute of Bangladesh. Md. Sadiqur Rahman, from the institute of Bangladesh. Laila Jarin, from the institute of Bangladesh. Rasheda Yasmin Shilpi, from the institute of Bangladesh . Rokibul Islam, from the institute of Bangladesh. and Md. Ataur Rahman, from the institute of Bangladesh. wrote a Research Article about,  Isolation and Identification of Bacterial Pathogens in Infected Shing Fish (Heteropneustes fossilis) from Freshwater Ponds in Bangladesh. entitled, Detachment, distinguishing proof of bacterial pathogens from infected Shing (Heteropneustes fossilis) cultured in freshwater ponds in Bangladesh. This research paper published by the International Journal of Biomolecules and Biomedicine (IJBB).  an open access scholarly research journal on Biomolecules. under the affiliation of the International Network For Natural Sciences | INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Among the local fishes, Shing (Heteropneustes fossilis) is one of the most demandable, popular and highly valuable fish in Bangladesh. A total of 84 clinically infected shing fishes were directly collected by a cultivator from their own ponds between April 2019 and December 2019. In total, eighty four fish-based ponds, 58(69.1%) were in Mymensingh region and the rest 26(30.9%) were in Netrakona districts in Bangladesh. Out of 84 infected fish samples, 74(88.1%) were infected with pathogenic bacteria and 10(11.9%) were with normal flora. A total of 74 pathogenic bacterial strains were isolated and among the isolates Aeromonas spp, Pseudomonas spp, Staphylococcus spp, Citobacter spp, and Vibrio spp, appeared to be the main pathogen in the diseased fishes. Among the isolated species of bacteria distribution of the largest pathogens Aeromonas species was 38 (51.4%), and second the largest Pseudomonas spp was 15(20.3%). The rest of isolates were distributed as Staphylococcus spp 7(9.4%), Citobacter spp 4(5.4%), Vibrio spp 3(4.1%) and only 7(9.4%) others namely Bacillus spp, Edwardsiella spp, Enterococcus spp, Flavobacterium spp, Klebsiella spp in infected H. fossilis. The cultivation of shing (H. fossilis) fishes is dramatically increased all over the country. However, bacterial diseases may influence to decrease the production in ponds water. In this work, bacterial pathogens were sensitive against Ciprofloxacin (77%), Cotrimoxazole (97.3%), and Enorfloxacin (97.8%). All the strains showed resistant to 74/74(100%) Amoxicillin, and 63/74(85.1) erythromycin. The intermediate sensitive against Colistin was 35.1% and Doxycycline was 22.9% respectively. 

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Introduction

Stinging catfish (Heteropneustes fossilis) is an indigenous air-breeding catfishes of South-East-Asia which is locally named as Shing in various parts of Bangladesh. Shing (H. fossilis) is extremely wellknown and exceptionally important fish species in Bangladesh. In viewpoints, it isn't just perceived for its delightful taste and market esteem but at the same time is profoundly respected for being restorative and healthful. Due to high demand and market price, it is cultured in farms with high stocking density. Despite the fact that Shing (H. fossilis) culture has incredible potential in Bangladesh, different illnesses of Shing causes genuine financial misfortunes in view of their high mortality under cultivating conditions. Generally, different species of cultivated and freshwater fishes are infected by Aeromonas spp in Bangladesh (Sarker et al., 2000). Moreover, Rashid et al. (2008) distinguished A. hydrophila from epizootic ulcerative syndrome (EUS) influenced shing (H. fossilis). Once upon a time, shing was bounteously accessible in the vast water of Bangladesh, yet by and by, it is undermined due to abuse and different environmental changes in its regular natural surroundings. Despite the fact that, new approach of fry and fingerlings of shing fishes has been developed in recent years, but obscure diseases of shing (H. fossilis) cause great economic losses because of their high mortality rate. In any case, the production of H. fossilis is identified with their aquaculture credits which incorporate capacity to withstand taking care of pressure, ailment opposition, high development rate, fruitfulness and attractiveness (Anyanwu et al., 2014).

Microscopic organism associated to produce infections in fish species have been accounted in various locale of Bangladesh and the revealed microbes were Aeromonas hydrophila (Ahamad et al., 2013), Flavobacterium columnare in columnaris infection (Declercq et al., 2003), Edwardsiella spp in edwardsiellosis (Mohanty and Sahoo, 2007), Aeromonas salmonicida in run of the mill furunculosis and Psudomonas species (Austin, 2011). The dangerous microbes such as Pseudomonas species, Aeromonas species, Staphylococcus species, Flavobacterium species, Citobacter species Edwardsiella species, and Vibrio species that live in every pond causing perilous, bacterial disease, for example, ulcer, blade decay and tail spoil of fishes. In Bangladesh, there is minimal accessible literature about bacterial infected shing fishes and antimicrobial sensitivity patterns of the isolates that have not been accounted for to gather enough information on pond cultured shing fish diseases. Therefore, the current study was embraced to isolate and identify bacteria from the infected pond cultured shing (H. fossilis) and observe their antibiotic affectability against various anti-infection agents.

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Source : Detachment,distinguishing proof of bacterial pathogens from infected Shing (Heteropneustesfossilis) cultured in freshwater ponds in Bangladesh