Overcoming Somatic Embryogenesis Challenges in Cocoa with IM Medium | InformativeBD

Koné Daouda,  from the institute of the Côte d’Ivoire. Thiémélé Deless Edmond Fulgence, from the institute of the Côte d’Ivoire. Silué Oumar, from the institute of the Côte d’Ivoire .Yao Saraka Didier Martial, from the institute of the Côte d’Ivoire. And N’Nan Alla Oulo, from the institute of the Côte d’Ivoire. wrote a Research Article about, Overcoming Somatic Embryogenesis Challenges in Cocoa with IM Medium. Entitled, Use of Im medium to overcome recalcitrance to somatic embryogenesis of improved cocoa genotypes (Theobrama cacao L.). This research paper published by the International Journal of Biosciences (IJB). an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences | INNSpub. an open access multidisciplinary research journal publisher.

Abstract

In vitro recalcitrance is the inability of plant cells, tissues and organs to respond to tissue culture manipulations. In cocoa (Theobroma cacao L.), recalcitrance is a limiting factor in tissue culture for the production of somatic embryos. This study aims to improve the production of somatic embryos by In Vitro culture of improved genotypes deemed recalcitrant to somatic embryogenesis. ‘’Im’’medium was used to induce the production of embryos of four enhanced and recalcitrant somatic embryogenesis genotypes encoded C8, C14, C15 and C16. The plant material consists of explants of petals and staminodes. The best results, which are characterized by levels of cal embryogenesis and the number of somatic embryos of 16% and 07, respectively, were obtained in the C14 genotype. This study showed that it is possible to produce somatic embryos in these improved varieties, thus eliminating the recalcitrance of these genotypes to somatic embryogenesis. The availability of improved material consisting of identical plants will allow the creation of more homogeneous plantations with high yields. 

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Introduction 

The cocoa tree (Theobroma cacao L.) is a tree that comes from the Amazon basin. This plant with multiple virtues is mainly grown for its beans. They are the main raw material for the chocolate industry (Koné et al., 2021).

Despite its importance, cocoa farming is facing enormous difficulties, which can be explained by the attacks of several pathogenic and parasitic agents, the aging of the orchards, the high cost of inputs, the nonavailability, and the under-use of plant material. improved (Koné et al., 2021).

To overcome these difficulties, several methods have been considered. Conventional techniques for the multiplication of plant material, such as cuttings and grafting, have been proposed. Unfortunately, these vegetative horticultural propagation techniques have several drawbacks that do not allow their easy application (Koné et al., 2021). 

To overcome all these difficulties and obtain homogeneous material in abundant quantity, in vitro culture by somatic embryogenesis was considered. It is a method that makes it possible to produce a given genotype in sufficient quantity and in a homogeneous manner.

This method of in vitro propagation has advantages over conventional methods of propagation and largescale production of woody plants while ensuring stability and genetic integrity (Kouassi et al., 2017a; Eliane et al., 2019; Koné et al., 2019). Regenerators, i.e., plants regenerated from somatic embryos, in addition to being genetically identical to each other, behave like seedlings (Jane et al., 2017; Kouassi et al., 2018). 

However, this method presents some problems in the cocoa tree because of the recalcitrance of certain genotypes. This recalcitrance is reflected in the variation in the rate of somatic embryos from one genotype to another, often with very low or even zero somatic embryo rates. Overcoming this recalcitrance requires optimizing existing protocols or developing new ones. The works of Koné et al. (2019; 2021) have shown that mineral salts and carbon sources taken individually make it possible to resolve this recalcitrance. The general objective of this study is to test the effect of the Im medium on lifting the recalcitrance of recalcitrant genotypes to allow their production in sufficient quantity and their large-scale distribution.

Reference

Driver JA, Kuniyuki AH. 1984. In vitro propagation of paradox walnut root stock. HortScience 19, 507-509.

Eliane MT, Modeste KK, André SB, Edmond KK, Mongomaké K. 2019. Effect of Water Stress Induced by Polyethylene Glycol 6000 on Somatic Embryogenesis in Cocoa (Theobroma cacao L.). Agricultural Sciences 10, 1240-1254.

Garcia C, Furtado AA, Costa M. 2019. Abnormalities in somatic embryogenesis caused by 2,4-D: an overview. Plant Cell, Tissue and Organ Culture, 137(2), 193-212. http://dx.doi.org/10.1007/s11240-019-01569-8

Garcia C, Corrêa F, Findley S. 2016. Optimization of somatic embryogenesis procedure for commercial clones of Theobroma cacao L. African Journal of Biotechnology 15(36), 1936-1951. http://dx.doi.org/10.5897/ajb2016.15513

Jane K, Siaka K, Lucien D, Georges N. 2017. Enhanced plantlet regeneration in two cacao (Theobroma cacao) Clones from Immature Inflorescence Explants. HORTSCIENCES, 52(6), 892–895. http://dx.doi.org/10.21273/HORTSCI11844-17

Koné D, Kouassi KM, N’Nan AO, Kouablan KE. 2019. Induction of somatic embryos of recalcitrant genotypes of theobroma. Journal of Applied Biosciences 133, 13552–13560.

Koné D, Kouassi KM, N’Nan AO, Kouablan KE. 2019.  Use of mineral salts to remove recalcitrance to somatic embryogenesis of improved genotypes of cacao (Theobrama cacao L.). African Journal of Biotechnology 20(1), 33-42.

Koné D, Kouassi  KM, N’Nan AO, Kouablan KE. 2019. Carbon source and lifting of recalcitrance to the induction of somatic embryos in cacao (Theobroma cacao L.).International journal of biosciences 19(1), 75-84.

Kouassi KM, Manlé TE, Koné D, Soumahoro AB, Koné T, Kouablan KE, Koné  M. 2017a. Effect of antioxidants on the callus induction and the development of somatic embryogenesis of cocoa [Theobroma cacao (L.)]. Australian Journal of Crop Sciences 11(1), 25-31. https://doi.org/10.21475/ajcs.2017.11.01.pne174

Kouassi KM, Kahia J, Kouame NC, Tahi GM, Kouablan KE. 2017. Comparing the effect of plant growth regulators on callus and somatic embryogenesis induction in four elite Theobroma cacao L. Genotypes. Hortsciences 52(1), 142–145.  http://dx.doi.org/10.21273/HORTSCI110.92-16

Kouassi KM, Kouablan KE, Silué O, Tahi GM, Touré M, Konan KP. 2018.Comparison of systems combining auxins with thidiazuron or kinetin supplemented with polyvinylpirrolidone during embryogenic callus induction in three Theobroma cacao L. genotypes. Internatinal Journal Biological and Chemical Sciences 12(2), 804-811. https://doi.org/10.4314/ijbcs.v12i2.15

Sandra MMN, Ana MHR, Aura IUT. 2023. Propagation of the Colombian genotype of cacao (Theobroma cacao L.) CNCh-12 by somatic embryogenesis.  Revis Bionatura 8(1), 26. http://dx.doi.org/10.21931/RB/2023.08.01.16.bd268

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