Showing posts with label Escherichia coli. Show all posts
Showing posts with label Escherichia coli. Show all posts

Drinking Water Quality & Disease Risks in Rural Azad Kashmir | InformativeBD

Drinking water quality and risk of waterborne diseases in the rural mountainous area of Azad Kashmir Pakistan

Ali Akbar, Uzma Sitara, Shabir Ahmed Khan, Niaz Muhammad, Muhammad Iftikhar Khan, Yasir Hayat Khan, and Saeed Ur Rehman Kakar, from the institute of Pakistan. wrote a Research article about, Drinking Water Quality & Disease Risks in Rural Azad Kashmir. Entitled, Drinking water quality and risk of waterborne diseases in the rural mountainous area of Azad Kashmir Pakistan. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

A study was conducted to evaluate drinking water safety at three different levels including sources, system and household in district Bagh, an earthquake affected area of Azad Jammu and Kashmir Pakistan. Portable water testing kit (Oxfam-DelAgua) was used for the detection of thermo-tolerant fecal coliform (Escherichia coli). A total (n=254) number of drinking water samples were examined for the presence of fecal coliform. It was found that, 68.5% (174 out of 254) of the overall samples tested were contaminated with Escherichia coli. The 52% (40 out of 77) of the water samples at source level, whereas 69% (58 out of 84) of water sample collected from system and 71% (66 out of 93) at household level were found contaminated with Escherichia coli. It was concluded that unprotected drinking water sources, improper management of waste, vulnerable sewage and ignorance regarding health and hygiene are the main reason of water contamination in the area.

Submit your article to IJB Journal

Read more : Somatic Embryogenesis in Rosa hybrida from Leaf & Petiole Explants | InformativeBD 

Introduction

Water is an important component of human body and is the need of life (Muhammad et al., 2012). Presence of deleterious chemicals and pathogenic microbes can cause a serious health problem, leading to infections and death (Lima et al., 2005). The drinking water contaminated with any pathogenic bacteria is unsafe for human consumption and household use (Muhammad et al., 2012). Access to safe drinking water is not only the prime need for survival and health but is also basic human rights (WHO, 2000). Safety of drinking water remains an important public health concern particularly in emergency situations (Ferretti et al., 2010). Pathogens that cause diarrheal diseases are being linked with contaminated water consumption, such pathogens are the main cause of gastrointestinal infections. The childhood mortality rate due to diarrheal diseases is 2.5 million each year (Muhammad et al., 2012; Oswald et al., 2007). Each year approximately five million children die due to the use of unsafe water (Shar et al., 2010). Approximately 1.8 million kids died in developing countries (excluding china) caused by biological agents or microorganisms originating from food and water in year 1998 (Akbar and Anal 2011). Vulnerable and Unsafe drinking water supplies are contributing in high rate of human morbidity and mortality worldwide. It has been a national concern in United States of America to provide safe drinking water since before the 20th century (Sobsey 2006). Unprotected or protected communal water sources are the key means of potable water in many developing countries (Gundry et al., 2004; WHO, 2000). It has been estimated that 1/3 of the total world population use ground water for drinking purpose (Nickson et al., 2005). Obtaining safe water from a communal source remains a prime concern of the people in developing countries (Joyce et al., 1996). Vulnerable sewage and sanitation lines and direct discharge of waste to natural reservoirs and water bodies are the major cause of fecal contamination (Huttly, 1990).

A group of bacteria called coliform are the primary indicator of water pollution. The presence of these microbes is associated with the presence of disease causing microorganisms (Muhammad et al., 2013; Shar et al., 2010). Bacteriological examination of water samples are usually undertaken to estimate the water quality. Most of the waterborne disease is related to faecal pollution of water sources. Therefore water microbiology is largely based on the need to identify indicators of faecal pollution such as Escherichia coli (Barrell et al., 2000). The detection and enumeration of coliform bacteria for the evaluation of microbiological drinking water quality has been exist from since 1912 (Bancroft et al., 1989). Drinking water should be pathogens free and with good organoleptic characteristics (Nevondo and Cloete, 1999). Contamination of drinking water during or after the collection from a safe source has been identified as a problem in rural areas particularly. A vast variety of pathogens are associated with water to cause severe diseases such as diarrhea, Poliomyelitis and hepatitis. The microbes such as, Vibrio cholera, Rotavirus, Astrovirus, Cryptosporidium, Gardia, Entameoba histolytica, Shagella etc are important pathogens, can cause severe damage to human health associated to unsafe water (Gundry et al., 2004; Hamer et al., 1998). In developing countries huge population are suffering from health problems associated with unavailability of drinking water or contaminated drinking water (Leeuwen, 2000).

In Pakistan it has been estimated that 40% of all deaths and 30% of all diseases is associated to unsafe water. Every fifth person suffers from illness due to polluted water. It has been estimated that three million Pakistanis suffer, while 0.1 million die from waterborne diseases annually (Haydar et al., 2009). It has been estimated that 44% of the Pakistani population does not have access to safe drinking water, while the figures rise to 80 percent in rural area population. Almost 1.1 billion people are lacking adequate access to water, whereas 2.4 billion people live without ample sanitation (Rosemann, 2005). The objective of this study was to evaluate and highlight the bacteriological quality of drinking water at three different levels such as, sources, water supply system and household and possible exposure to the pathogenic bacteria during daily consumption of water in the remote mountainous area of Azad Kashmir Pakistan.

Study area The study conducted in different areas of district Bagh, Azad Jammu and Kashmir Pakistan. Topography of the study area is mountainous and majority of the people used to live in small scattered villages dependent on natural springs particularly well and flowing water channels generally as their primary source of water (drinking and household use). The area was severely affected by October, 2005 earthquake (Figure1). In the current study a detail survey regarding the existing drinking water quality of the area in term of microbiological contamination was conducted.

Reference

Akbar A, Anal KA. 2011. Food safety concerns and food-borne pathogens, Salmonella, Escherichia coli and Campylobacter. FUUAST Journal of Biology 1(1), 5-17.

Bancroft K, Nelson ET, Childers GW. 1989. Comparison of the presence-absence and membrane filter techniques for coliform detection in small, non-chlorinated water distribution systems. Applied and Environmental Microbiology 55(2), 507-510.

Barrell RAE, Hunter PR, Nichols G. 2000. Microbiological standards for water and their relationship to health risk. Communicable Diseases and Public health 3(1), 8-13.

Ferretti E, Bonadonna L, Lucentini L, Libera SD, Semproni M, Ottaviani M. 2010. A case study of sanitary survey on community drinking water supplies after a severe (post-Tsunami) flooding event. Annali dell’Istituto Superiore di Sanità 46(3), 236-241. http://dx.doi.org/10.4415/ANN_10_03_03

Gundry S, Wright J, Conroy R. 2004. A systematic review of the health outcomes related to household water quality in developing countries. Journal of Water and Health 2(1), 1-13.

Hamer DH, Simon J, Thea DM, Keusch G. 1998. Childhood diarrhoea in Sub-Saharan Africa, Harvard University, Harvard. 1-32.

Haydar S, Arshad M, Aziz JA. 2009. Evaluation of drinking water quality in urban areas of Pakistan: A case study of southern Lahore. Pakistan Journal of Engineering and Applied Science 5, 16-23.

Huttly SR. 1990. The impact of inadequate sanitary condition on health in developing countries. World Health Statistics Quarterly 43, 118-126.

Joyce TM, Meguigan KG, Elmore-Meegan M, Conroy RM. 1996. Inactivation of fecal bacteria in drinking water by solar heating. Journal of Applied and Environmental Microbiology 62(2), 399-402.

Kress M, Gifford G. 1984. Fecal coliform release from cattle fecal deposits. Water Recourses Bulletin 20(1), 61-66. http://dx.doi.org/10.1111/j.17521688.1984.tb04642.x

Leeuwen FXR Van. 2000. Safe drinking water; the toxicologist’s approach. Food and Chemical Toxicology 38, 51-58. http://dx.doi.org/10.1016/S0278-6915(99)00140-4

Lieverloo JHM van, Blokker JME, Medema G. 2007. Quantitative microbial risk assessment of distributed drinking water using faecal indicator incidence and concentrations. Journal of Water Health 5(1), 131-149. http://dx.doi.org/10.2166/wh.2007.134

Lima JRO, Marques SG, Gonçalves AG, Filho NS, Nunes PC, Silva HS, Monteiro SG, Costa JML. 2005. Microbiological analyses of water from hemodialysis services in são luís, maranhão. Brazil. Brazilian Journal of Microbiology 36, 103-108. http://dx.doi.org/10.1590/S1517838220050002000 01

Muhammad F, Ikram M, Khan S, Khan K, Shah SH, Badshah Z, Ahmad W, Shah SN. 2013. Flood disaster in Charasadda, Pakistan: Bacteriological examination of drinking water. International Journal of Biosciences 3(5), 51-59. http://dx.doi.org/10.12692/ijb/3.5.51-59

Muhammad N, Bangush M, Khan AT. 2012. Microbial contamination in well water of temporary arranged camps: A health risk in northern Pakistan. Water Quality Exposure and Health 4(4), 209-215. http://dx.doi.org/10.1007/s12403-012-0080-0

Nevondo TS, Cloete TE. 1999. Bacterial and chemical quality of water supply in the Dertig village settlement. Water SA 25(2), 215-220.

Nickson RT, McArthur JM, Shrestha B, Kyaw-Myint TO, Lowry D. 2005. Arsenic and other drinking water quality issues, Muzaffargarh district, Pakistan. Applied Geochemistry 20, 55-68. http://dx.doi.org/10.1016/j.apgeochem.2004.06.004

Oswald WE, Lescano AG, Bern C, Maritza MC, Lilia C, Robert HG. 2007. Fecal contamination of drinking water within Peri-urban households, Lima, Peru. The American Journal of Tropical Medicine and Hygiene 77(4), 699-704.

Oxfam-DelAgua. 2004. Oxfam-DelAgua portable water testing kit, users manual (version 4.1), revised, Univ. of Surrey, Marlborough, U.K.

Rosemann N. 2005. Drinking water crisis in Pakistan and the issue of bottled water the case of Nestlé’s ‘Pure Life’. Swiss coalition of development organizations Swissaid, catholic lenten fund bread for all, Helvetas Caritas, Interchurch aid, 1-37.

Shar AH, Kazi YF, Kanhar NA, Soomro IH, Zia SM, Ghumro PB. 2010. Drinking water quality in Rohri City, Sindh, Pakistan. African Journal of Biotechnology 9(42), 7102-7107. http://dx.doi.org/10.5897/AJB10.410

Sobsey DM. 2006. Drinking water and health research: a look to the future in the United States and globally.  Journal  of  Water  Health  4(1),  17-21. http://dx.doi.org/10.2166/wh.2005.035

Tariq MI. 2013. Pakistan sanitation country paper. Cabinet   secretariat,   climate   change   division, government of Pakistan, Islamabad, 44000, Pakistan.

World   Health   Organization   and   United Nations  Children’s  Fund.  2000.  Global  water supply  and  sanitation  assessment  (report),  New York/Geneva. 1-80.

Article source : Drinking water quality and risk of waterborne diseases in the rural mountainous area of Azad Kashmir Pakistan   

 

Blue Butterfly Pea: Phytochemicals and E. coli Inhibition | InformativeBD

Blue Butterfly pea plant.

Ferdinand A. Dumalagan, Caryl Joy Alvares, and Robert L. Salamasan, from the different institute of the Philippines, wrote a research article aboute, Blue Butterfly Pea: Phytochemicals and E. coli Inhibition, entitled, "Qualitative phytochemical analysis and inhibitory property of Blue Butterfly Pea flower extract against Escherichia coli".This research paper published by the International Journal of Biosciences | IJB, an open access scholarly research journal on Biosciences, under the affiliation of the International Network For Natural Sciences | INNSpub, an open access multidisciplinary research journal publisher.

Abstract

From ancient times, medicinal plants have been utilized to heal a variety of human illnesses. The development of antibiotics as a means of treating diverse bacterial illnesses sparked a revolution in medicine. Several therapeutic plant extracts are known to have antibacterial properties that are applied in the preservation of food and for therapeutic purposes. Blue butterfly pea (Clitoria ternatea) is a plant that is popularly used as medicine and recognized to treat a number of illnesses. Practically, every component of this plant is said to have therapeutic properties. Furthermore, C. ternatea plant has strong antibacterial effect as it contains biological compounds that act as best antimicrobial property against bacteria. Also, phytochemical analysis of C. ternatea flower extract showed the presence of flavonoids, steroids and tannins in which these phytochemical compounds contributed to the antibacterial ability of the flower extract. On the other hand, C. ternatea flower extract has a heavy amount of flavonoids and these results to a very active capacity to inhibit the growth of the Escherichia coli. Hence, flavonoids are reported that it has the capability against infectious degenerative disease, viral and bacterial. Certainly, this medicinal plant may be used for an alternative medicine considering the fact that this is harmless, low-cost production and its flower can be eaten raw. 

Submit your article to  IJB Journal

 Read more:  Bilaspur's Wild Mushrooms: Edible and Medicinal Diversity | InformativeBD

Introduction

Human illnesses are treated using medicinal plants and have been for a very long time. The development of antibiotics as a means of treating diverse bacterial illnesses sparked a revolution in medicine. However, their indiscriminate usage has caused an alarming growth in the number of microorganisms that are resistant to antibiotics, giving rise to multiresistant strains, which has raised concerns around the world (Shariff, 2001).

Clitoria ternatea L. is a species of Clitoria under Fabaceae family is often utilized as a traditional plant called as blue butterfly pea. It is Ayurvedic medicinal plant as a memory booster, stress reliever, and calming, antidepressant, anticonvulsant, and anxiolytic also a sedative (Ramkissoon et al. (2013). More so, C. ternatea has been utilized for a number of medical conditions. Uses for its roots include cure heartburn, bloating, constipation, fever, arthritic pain, sore throat, while its seeds are utilized as a treatment for eye and skin conditions, to treat colic and swollen joints with a laxative. The conventional Cuban culture employs either a single root decoction or paired with flowers to induce menstruation and encourage as well as to treat liver and intestinal problems, uterine contractions problems (Mukherjee et al., 2008; Fantz 1991). Furthermore, its flower petal's nutritional value using anthocyanins as a naturally occurring blue coloring in a various cuisine. It is also act as anti-oxidant, antibacterial, anti-inflammatory, and other pharmacological properties that are present in the extract of C. ternatea (Gupta et al., 2010).

Moreover, a large and increasing number of patients in the world used medicinal plants and herbs for health purposes. There are hundreds of biologically active compounds developed from traditional medicinal plants (Inoue, 2018). It is believed that the plant’s medicinal crude extract is more biologically active that isolated compounds due to its synergistic effects (Jana and Shekhawat, 2010).

Based on the presented prior arts that Clitoria ternatea possess medicinal values and clearly benefit the users. This study explores on the screening of the antibacterial activity of phytochemical properties present in blue butterfly pea that cited that contains antibacterial composition against Escherichia coli. Hence, it can be an alternative medicine to cure various diseases that acts as anti-viral, antiinflammatory, anti- allergic, has rich in antioxidants and good treatment for mental illness that contributes to the range of rediscovering essential uses of this plant.

Reference

Alok S, Gupta N, Malik A. 2015. International Journal of Life Sciences Rev 1(1), 1-9.

Chusak C, Thilavech T, Henry CJ, Adisakwattana S. 2018. Acute effect of Clitoria ternatea flower beverage on glycemic response and antioxidant capacity in healthy subjects: A randomized crossover trial. BMC Complementary and Alternative Medicine 18, 1-11. https:// doi.org/ 10.1186/s12906-017-2075-7.

Fantz PR. 1991. Ethnobotany of Clitoria (Leguminosae). Econ Bot 45, 511-520. https:// doi.org /10.1007/BF02930715.

Ferreira D, Gross GG, Hagerman AE, Kolodziej H, Yoshida T. 2008. Tannins and related polyphenols: Perspectives on their chemistry, biology, ecological effects, and human health protection. Phytochemistry 69, 3006-3008.

Gupta GK, Chahal J, Bhatia M. 2010. Old and new aspects. Journal on Pharmacy Research 3, 2610-4.

Haditio SM, Muttaqin Z, Hadi L. 2021. Comparison of Inhibition Zones between Butterfly Pea flower (Clitoria ternatea) and Lemongrass (Cymbopogon citrarus) against Streptococcus mutans and Staphylococcus aureus. Biomedical Journal of Indonesia 7, 374-378. https:// doi.org/ 10.32539/bji.v7i2.313.

Harborne, JB and Williams, CA. 2000. Advances in Flavonoid Research since 1992. Phytochemistry 55, 481-504.

Jamil N, Pa’ee F. 2018. AIP Conference Proceedings 2002, 020044

Kamilla L, Mnsor SM, Ramanathan S, Sasidharan S. 2009. Antimicrobial activity of Clitoria ternatea (L.) extracts. Pharmacologyonline 1:

Ke S. 2018. Recent Progress of Novel Steroid Derivatives and Their Potential Biological Properties. Mini-Rev. Med. Chem. 18, 745-775.

Kumar MN, More D. 2019. Phytochemical analysis and bioactivity of selected medicinal plant of butterfly-pea (Clitoria ternatea L.) used by Kolam tribe Addjoing region of Telangana and Maharashtra states. The Pharma Innovation Journal 8, 417-421.

Lakshan SAT, Jayanath NY, Abeysekera WPKM, Abeysekera WKSM. 2019. A commercial potential blue pea (Clitoria ternatea L.) flower extract incorporated beverage having functional properties. Evidence-based Complementary and Alternative Medicine 2019: e2916914. https://doi.org/10.1155 /2019/2916914

Lijon MB, Meghla NS, Jahedi E, Rahman MA, Hossain I. 2017. Phytochemistry and pharmacological activities of Clitoria ternatea. International Journal of Natural and Social Sciences 4, 1-10.

Mahomoodally MF, Gurib-Fakim A, Subratty AH. 2005. Antimicrobial activities and phytochemical profles of endemic medicinal plants of Mauritius. Pharmaceut Biol 43, 237-242

Mukherjee PK, Kumar V, Kumar NS. 2008. The Ayurvedic medicine Clitoria ternatea-From traditional use to scientific assessment. J Ethnopharmacol 120, 291-301. https://doi.org/10. 1016/j.jep.2008.09.009.

Okwu DE, Josiah C. 2006. Evaluation of the Chemical Composition of Two Nigerian Medicinal Plants in African Journal of Biotechnology 5, 357-361.

Pandey AK. 2007. Anti-staphylococcal activity of a pan-tropical aggressive and obnoxious weed Parihenium histerophorus: an in vitro study. Natl Acad Sci Lett 30, 383-386

Pratap Gowd MJS, Manoj Kumar M, Sai Shankar A, Sujatha B, Sreedevi E. 2012. Evaluation of three medicinal plants for anti-microbial activity. AYU (An International Quarterly Journal of Research in Ayurveda) 33, 423-428. https://doi.org/10.4103/0974-8520.108859.

Ramkissoon JS, Mahomoodally MF, Ahmed N, Subratty AH. 2013. Antioxidant and anti-glycation activities correlates with phenolic composition of tropical medicinal herbs. Asian Pacific J Tropical Medicine 6, 561-9.

Shariff ZM. 2001. Modern Herbal Therapy for Common Ailments. Nature Pharmacy Series Vol. I. 1st Edn, Spectrum Books Limited, Ibandan, Nigeria in Association with Safari Books (Export), Limited UK pp.9-84.

Uma B. 2009. Phytochemical analysis and antimicrobial activity of Clitoria ternatea Linn. against extended spectrum beta lactamase producing enteric and urinary pathogens. Asian J. Pharm. Clin. Res 2, 94-96.

Vu TT, Kim H, Tran VK, Vu HD, Hoang TX, Han JW, et al. 2017. Antibacterial activity of tannins isolated from Sapium baccatum extract and use for control of tomato bacterial wilt. PLoS One 12:e0181499. https://doi.org/10.1371/journal. pone.

Widyarman AS, Sumadi S, Agustin TP. 2018. Antibiofilm effect of Clitoria ternatea flower juice on Porphyromonas gingivalis in vitro. Journal of Indonesian Dental Association 1, 7-12. https://doi. org/10.32793/jida.v1i1.288.

 SourceQualitative phytochemical analysis and inhibitory property of Blue Butterfly Pea flowerextract against Escherichia coli