Growth Performance of Ceratocystis manginecans on Different Media | InformativeBD

Kokab Jabeen, from the institute of Pakistan and Shahzad Asad from the institute of Pakistan, wrote a Research article about, Growth Performance of Ceratocystis manginecans on Different Media. Entitled, Assessment of selected culture media for competent growth characteristics of Ceratocystis manginecans (Cause of Mango Sudden Death). This research paper published by the Journal of Biodiversity and Environmental Sciences | JBES. an open access scholarly research journal Biodiversity. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Comprehensive and critical facts of aspects which influence patterns of growth and nutrition of the fungi is precondition for research related to the relationship of host and pathogen. A number of media compositions likewise influence the colony morphology of fungus. Most conjoint and distressing disease in mango trees is Mango sudden death (MSD) caused by Ceratocystis manginecans. Present study is aimed to evaluate the influence of selected six different culture media (agar and broth media) on sporulation; colony characteristics and growth of the C. manginecans isolated from MSD infected mango trees. Statistical analysis of the data gathered revealed that all the treatments were significantly different from each other. Among solid media, Malt Extract Agar and Carrot Juice Agar supported best growth of C. manginecans89.53mm and 85.31mm respectively. Among broth media Carrot Juice supported better growth (503.33mg dry weight) than other media. The Characteristics of growth like color of substrate, colony color and margins, mycelia topography as well as sporulation of the C. manginecans were studied. Best sporulation of C. manginecans was shown in Malt Extract Agar media. Selection of most effective culture media for efficient growth characteristics of C. manginecans is necessary for its physiological and taxonomical study as well as for suitable disease management strategies.

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Introduction 

Mango sudden death (MSD) is menace to costeffective farming of mango. MSD cause drastic reduction in the production quantity and quality of mango. Early visible disease symptoms include bark splitting; gummosis from bark; vascular discoloration; and streaking underneath gummosis. Diseased mango tree leaves wither out, but stay attached with the tree. When the severely infected trunk is scrapped, rotted cankers are produced or exudation of foul smelled liquid occurs in some cases (Masood et al., 2010).

In Pakistan (2007) a wilt epidemic of Magnifier indica (mango trees) was stated. According to Wyk et al. (2007) and Asma et al. (2014), the confirmed pathogen of mango sudden death in Pakistan was Ceratocystis manginecans. The name Ceratocystis manginecans was derived from a Latin word neck means “to kill, slay/put to death”. This refers to the fact that C. manginecansis responsible for a serious disease of the mango trees. Colony colure of C. manginecansis grayish olive. It has banana odor (Wyk et al., 2007).

Foran in vitro study, fungus is isolated as pure culture in a particular media for the studies relating physiology, nutrition, growth as well as management of that particular fungus. A wide range of media can help in the isolation, the diametric growth, dry weight growth and sporulation of the fungus. Several media compositions can also influence the diverse colony morphology of fungus. A classical approach to distinguish any fungal species is morphological characterization, which is one of the foremost requisite of the fungal taxonomy (Diba et al., 2007).

Carbohydrates exist in plants in both forms (simple and complex).Before utilization, fungi convert carbohydrates of complex forms into simpler one (water soluble and low molecular weight sugars). Different fungal species retort differently to a specific compound. Fungi have noticeable variation in consumption of varied carbohydrate sources (Zain et al., 2009).

Comprehensive and critical information of aspects which influence patterns of growth and nutrition of the fungi is precondition for research related to the relationship of host and pathogen. A number of media compositions likewise influence the colony morphology of fungus. For growth parameters of a pathogen, much attention is not given to culture media (Mishra and Mishra, 2012).

Present study is aimed to evaluate the influence of selected o6 different culture media on sporulation; colony characteristics and growth of the C. manginecans isolated from MSD infected mango trees. Selection of most effective culture media for efficient growth characteristics of C. manginecans is necessary to be developed for suitable disease management strategies. It may aid to the physiological and taxonomical traits of C. manginecans.

Reference

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Khuhro RD, Nizamani SM, Abbasi QD, Solangi GS, Jiskani MM. 2005. Mango Tree Mortality due to Asian ambrosia beetle, Xylosandrus crassiusculus Mot. (Coleoptera: Scolytidae) Pak. J. Agri. Agril. Engg., Vet. Sc 21(1), 39-42.

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Article source : Assessment of selected culture media for competent growth characteristics of Ceratocystis manginecans(Cause of Mango Sudden Death)