Boosting Buck Fertility: Moringa Leaf Extract and Frozen Semen Quality in Boer Goats | InformativeBD

Muhammad Riyadhi,  Nursyam Andi Syarifuddin,  Muhammad Thahir, and Muhammad Rizal, from the  different institute of Indonesia, wrote a Research Article about, Boosting Buck Fertility: Moringa Leaf Extract and Frozen Semen Quality in Boer Goats. Entitled, The Effect of Addition of Moringa Leaf Extract in Lactose Extender on Frozen Semen Quality of Boer Crossbreed Goat. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Moringa leaves contain various nutrients that can protect spermatozoa during semen cryopreservation. This study aimed to examine the effect of moringa leaf extract in tris extender on the quality of frozen semen of Boer crossbreed goat. Semen was collected with an artificial vagina. Fresh semen was divided into four tubes and diluted with 73% tris extender + 20% egg yolk + 7% glycerol (control), 71% tris extender + 20% egg yolk + 7% glycerol + 2% moringa leaf extract (MLE-2), 69% tris extender + 20% egg yolk + 7% glycerol + 4% moringa leaf extract (MLE-4), and 67% tris extender + 20% egg yolk + 7% glycerol + 6% moringa leaf extract (MLE-6), respectively. Diluted-semen was loaded in a mini straw and then stored in a liquid nitrogen container for seven days. The quality of spermatozoa including motility, viability, and intact plasma membrane (IPM) were observed after diluting and thawing. The result showed that the addition of moringa leaf extract into the tris extender could improve the spermatozoa quality of frozen semen of Boer crossbreed goat. At the stage after thawing, the percentage of motility, percentage of viability, and percentage of IPM of MLE-4 (52.2, 65.2, and 65.0%) were significantly higher (p<0.05) compared to the control (43.7, 53.5, and 53.7%). It can be concluded that the addition of 4% moringa leaf extract is the appropriate concentration in producing frozen semen of Boer crossbreed.

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Introduction

One method to improve the performance of Indonesian local goats is to cross them with Boer goats through the application of artificial insemination (AI) technology. An important factor in the success AI is the quality of semen processing (Tamoes et al., 2014). The quality of semen processing can be maintained by adding various additive compounds to the semen extender, including moringa leaf extract. This is because moringa leaves contain high antioxidants (Kasolo et al., 2010) and antibacterial (Das et al., 2012).

Compounds that are antioxidants can inhibit the work of free radicals in damaging the plasma membrane of spermatozoa during semen preservation (Sitepu et al., 2018), to maintain the quality of spermatozoa. Moringa leaves contain flavonoid compounds that can bind free radicals (Kumala et al., 2016).

Improved semen quality preserved with an extender supplemented with moringa leaf extract was reported in bovine spermatozoa (Sokunbi et al., 2015) and Landrace pig spermatozoa (Fafo et al., 2016).

This study aims to examine the effect of various concentrations of moringa leaf extract in tris extender on the quality of frozen semen of Boer crossbreed goat. It is expected that the compounds contained in moringa leaf extract can protect against spermatozoa cell damage during the cryopreservation process so that the quality can be maintained and meet the requirements for use in the AI program.

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Article source : The Effect of Addition of Moringa Leaf Extract in Lactose Extender on Frozen Semen Quality of Boer Crossbreed Goat