Radiation and Roots: How Gamma Rays Affect Jatropha Seed Germination | InformativeBD

Fatimah, Agung Nugroho, Yuspihana Fitrial, and Rukmini, from the different institute  of Indonesia. wrote a Research Article about, Radiation and Roots: How Gamma Rays Affect Jatropha Seed Germination. Entitled, Comparison of Aspergillus niger and Rhizopus oryzae growth on Nymphaea pubescens seed culture media. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract 

Potato Dextrose Agar (PDA) is a commonly used medium for the growth of Rhizopus oryzae and Aspergillus niger. The purpose of the study was to determine Rhizopus oryzae and Aspergillus niger growth in the fermented process of Nymphaea pubescens unhulled seed. The hull of Nymphaea pubescens seed consists mostly of lignin and cellulose. The fungi that can grow in the Nymphaea pubescens seed fermentation process are expected to accelerate the Nymphaea pubescens seed dehulling process. This research is an experimental study with the initial stages of conducting a qualitative test of cellulolytic activity of Rhizopus oryzae and Aspergillus niger. Then inoculate Rhizopus oryzae and Aspergillus niger on PDA media and cultivate at Nymphaea pubescens unhulled seed. Observation of extracellular cellulases for the formation of clear zones around the cellulase-producing colonies in the cellulolytic qualitative test of Rhizopus oryzae and Aspergillus niger. The macroscopic observations of the growth hyphae of Rhizopus oryzae and Aspergillus niger. The results showed that Aspergillus niger has cellulolytic activity and can grow in the Nymphaea pubescens unhulled seed. But Rhizopus oryzae did not have cellulolytic activity and cannot grow in the Nymphaea pubescens unhulled seed at fermentation process.

Read more : Boost or Barrier? How 60Co Gamma Radiation Influences Jatropha Seed Germination | InformativeBD

Introduction

Nymphaea pubescens seeds have long been used by the people of wetland areas in Indonesia (Fatimah et al., 2022) and several countries (Aliyu et al., 2017) as an ingredient in the manufacture of traditional foods. Nymphaea pubescens seed has the main content of carbohydrates, proteins (Aliyu et al., 2017), and phytochemical components that have antioxidant activity (Aliyu et al., 2018). Nymphaea pubescens seeds consist of three main parts, namely macrosclereids, osteosclereids, and endosperm. Lignin and cellulose are the main components of the macrosclereids part of the grain.

Fungi have specific enzymatic for metabolism, including breakdown of the materials for organism growth. Rhizopus oryzae and Aspergillus niger commonly use Potato Dextrose Agar (PDA) media for cultivation growth. Pre-treatment dehulling using enzymes have been used in several grains, including proteases (Sreerama et al., 2009), xylanase, pectinase, and cellulase (Dabhi et al., 2019; Murumkar et al., 2016). The purpose of the study was to determine whether Rhizopus oryzae and Aspergillus niger could grow in the fermented process of Nymphaea pubescens unhulled seeds. The fungi that can grow in the Nymphaea pubescens seed fermentation process are expected to accelerate the Nymphaea pubescens seed dehulling process.

Reference

Aliyu M, Atiku MK, Abdullahi N, Imam AA, Kankara IA. 2018. Evaluation of In vitro Antioxidant Potentials of Nymphaea lotus and Nymphaea pubescens Seed Oils. International Journal of Biochemistry Research & Review 24, 1-8.

Aliyu M, Atiku MK, Abdullahi N, Zaharaddeen A, Imam AA. 2017. Comparative Evaluation of Nutritional Qualities of Nymphaea lotus and Nymphaea pubescens Seeds. International Journal of Biochemistry Research & Review 19, 1-10.

Amadioha AC. 1993. Production of Cellulolytic Enzymes by Rhizopus Oryzae in Culture and Rhizopus-Infected Tissues of Potato Tubers. Mycologia 85, 574-578.

Dabhi MN, Sangani VP, Rathod PJ. 2019. Effect of enzyme pretreatment on dehulling, cooking time and protein content of pigeon pea (variety BDN2). Journal of Food Science and Technology 56, 4552-4564.

Fatimah, Fitrial Y, Rukmini, Nugroho A. 2022. Effect of grain moisture on dehulling of Nymphaea pubescens seed. IOP Conference Series: Earth and Environmental Science 976, 012056.

Kusumawati N, Wardani AK, Zubaidah E, Sumarlan SH. 2020. Isolation, screening and identification of potential cellulolytic and xylanolytic mold from oil palm waste. IOP Conference Series: Earth and Environmental Science 475, 012083.

Murumkar RP, Borkar PA, Munje SS, Rathod PK, Rajput MR, Dhoke SM. 2016. Effect of enzyme pre-treatments on milling of pigeonpea. International Journal of Science, Environment and Technology 5, 23.

Sreerama YN, Sashikala VB, Pratape VM. 2009. Effect of enzyme pre-dehulling treatments on dehulling and cooking properties of legumes. Journal of Food Engineering 92, 389-395.

Article source : Comparison of Aspergillus niger and Rhizopus oryzae growth on Nymphaea pubescens seed culturemedia