Enhancing Garcinia kola Seed Germination Techniques | InformativeBD

Germination of Garcinia kola (heckel) seeds in response to seed sectioning, chemical pretreatment and different temperatures

Joseph M. Asomaning, from the institute of Ghana. wrote a Research article about, Enhancing Garcinia kola Seed Germination Techniques. Entitled, Germination of Garcinia kola (heckel) seeds in response to seed sectioning, chemical pretreatment and different temperatures. This research paper published by the Journal of Biodiversity and Environmental Sciences | JBES. an open access scholarly research journal Biodiversity. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Garcinia kola Heckel is a multi-purpose tree widely used in West Africa resulting in its over-exploitation, the species is extinction-threatened. On farm conservation through cultivation has been recommended. However, seeds of the species can take about 18 months to germinate. This study aims at generating information on how the combinations of the techniques of seed sectioning, chemical treatment and temperature can be used to enhance germination of the seed. Seed sections and whole seeds pretreated with dormancy breaking chemicals were germinated on a gel of 1% water agar at 20, 25, 30 and 35°C. Statistical design used in the investigation was a completely randomized design in a 5×7×4 factorial (germination materials × chemical treatments × temperature). Germination data showed significances (p<0.001) namely: germination materials, germination temperatures, germination materials x germination temperatures, germination materials x chemicals, germination temperatures x chemicals.

Submit your article to JBES Journal

Read more : Optimizing In Vitro Organogenesis in Sugarcane Using Plant Growth Regulators | InformativeBD 

Introduction

Garcinia is a tropical plant genus including several species in Africa, America and Asia. These species are commonly useful for many purposes. The seeds of G. kola have pharmacological uses in treating coughs, throat infections, bronchitis and hepatitis (Farombi et al., 2005) The seeds which serve as a bitter stimulant also serve as snake repellent when they are placed round the compound (Nair, 1990). Other medicinal uses include: purgative, antiparasitic, antimicrobial. The seeds are used to prevent and relieve colic, cure head or chest colds.

This plant has shown bronchodilator effect (Orie and Ekon 1993), anti-inflammatory, antimicrobial, antibacterial and antiviral properties (Akoachere et al., 2002). In laboratory tests, Garcinia kola was found to halt the deadly disease caused by Ebola virus in its tracks. The virus causes Ebola hemorrhagic fever - an often-fatal condition (Anonymous, 1999). Compounds from the plant have also proved effective against some strains of flu, a contagious respiratory disease also commonly known as influenza (Iwu, 1993). Its by-products are also useful: the wood makes excellent fuel wood; its dense rounded crown makes it an ideal tree for shade around homestead; the branches are used as chewing stick because of its bitter taste and antibacterial activities of its extracts (Taiwo et al., 1999). The bark of the stem is used in the tanning and dyeing industry (Irvine, 1961).

Because of its high interest resulting in its overexploitation, Garcinia kola is extinction-threatened in several West and Central African countries such as Ivory Coast (FAO (1996), Ghana (Wong, 1997), Congo and Cameroon (Tchatat, 1999). It is therefore useful to undertake on farm conservation by small holder farmers through agroforestry systems in order to decrease the pressure on wild population of the species. However, the major difficulty in Garcinia kola propagation as for several species of Garcinia genus is related to seeds germination. Due to dormancy in Garcinia, seeds can take as long as 18 months to germinate (Aduse-Poku et al., 2003). Some studies to investigate seed germination of some species in the genus Garcinia has been done at the farmer’s level and under laboratory conditions. These include Garcinia gummi-gutta (Geeta et al, 2006); Garcinia indica (Malik et al., 2005); Garcinia kola (Agyili et al., 2007; Kanmegne and Omokolo 2008).

The present work aims at generating information on how the combination of the techniques of seed sectioning, chemical treatment and temperature can be used to enhance the germination of G. kola. The information will be useful in raising large quantities of seedlings for several farming communities in Ghana who are being encouraged to plant the species on their cocoa farms as a shade crop and as a means of conserving the species.

Reference

Aduse-Poku K, Nyinaku F, Atiase VY, Awuah R, Mensah EO, Nyantakyi D, Owusu HK and Agyenim-Boateng B. 2003. Improving rural livelihoods within the context of sustainable development: case study of Goaso forest district. Tropenbos International Ghana, University of Amsterdam and Institute of Renewable Natural Resources 50 pp.

Agyili J, Sacandé M, Koffi E and Peprah T. 2007. Improving the collection and germination of West African Garcinia kola Heckel seeds. New Forests 34, 269-279.

Akoachere JF, Ndip RN, Chenwi EB, Ndip LM, Njock TE and Anong DN. 2002. Antibacterial effect of Zingiber officinale and Garcinia kola on respiratory tract pathogens. East Africa Medical Journal 79(11), 588-592.

Anegbeh PO, Iruka C and Nkirika C. 2006. Enhancing germination of bitter cola (Garcinia kola) Heckel: prospects for agroforestry farmers in the Niger Delta. Scientia Africana 5(1), 1118-1931.

Asomaning JM, Olympio NS, Sacandé M. 2011. Desiccation sensitivity and germination of recalcitrant Garcinia kola Heckel seeds. Research Journal of Seed Science 4(1), 15-27.

Asomaning JM. 2009. Seed desiccation tolerance and germination of seven important forest tree species in Ghana. Thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy. School of Graduate Studies, Department of Horticulture. Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana 1-187 .

Choudhary DK. 1975. Causes of poor and erratic germination in Atropa belladonna. Planta Med 27, 18-22. DOI: 10. 1055/s-0028-1097754.

Daws ML, Gaméné SC, Sacandé M, Pritchard HW, Groot PCG and Hoekstra F. 2004. Desiccation and storage of Lannea microcarpa seeds from Burkina Faso. pp 32-39.

Eyog-Matig O, Aoudji AKN, Linsouss C. 2007. Garcinia kola Heckel seeds dormancy-breaking. Applied Ecology and Environmental Research 5(1), 63-71.

Farombi EO, Adepoju BF, Ola-Davies OE and Emerole GO. 2005. Chemoprevention of aflatoxin B1-induced genotoxicity and hepatic oxidative damage in rats by kolaviron, a natural bioflavonoid of Garcinia kola seeds. European Journal Cancer Prevention 14 (3), 207-214.

Food and Agriculture Organization, FAO. 1996. Côte d’Ivoire: Rapport de pays pour la conférence technique internationale de la FAO sur les ressources phytogénétiques. FAO 75 pp.

Geeta J, Kumar AH, Balakrishna G, Srinivisa YB. 2006. Production of supernumerary plants from seed fragments in Garcinia gummi-guta: evolutionary implications of mammalian frugivory. Current Science 91(3), 372-376.

International Seed Testing Association, ISTA. 1999. International rules for seed testing. Supplement to Seed Science and Technology 27, 1-133.

Irvine FR. 1961. Woody Plants of Ghana with Special Reference to their Uses. Oxford Univ. Press 143-144.

Malik SK, Chaudhury R, Kalia RK. 2005. Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Scientia Horticulturea 106, 539-553.

Nair PKR. 1990. The prospects for agroforestry in the tropics. Technical Paper 131. Nairobi, ICRAF.

Nzegbule E, Mbakwe R. 2001. Effect of pre-sowing and incubation treatment on germination of Garcinia kola (Heckel) seeds. Fruits 56(6), 437-442.

Of ori DA, Asomaning JM, Peprah T, Agyeman VK, Anjarwalla P, Tchoundjeu Z, Mowo JG, Jamnadass R. 2015. Addressing constraints in propagation of Allanblackia spp. through seed sectioning and air layering. Journal of Experimental Biology and Agricultural Sciences 3(1), 89-96.

Orie NN, Ekon EU. 1993. The bronchodilator effect of Garcinia kola. East Africa Medical Journal 70(3),143-145.

Rai ND. 2003. Human use, reproductive ecology and life history of Garcinia gummi-guta : a non-timber forest product, in the western Gaths, India. Thesis submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy. Pennsylvania State University, Graduate School, Department of Biology 191 pp.

Smith MT, Wang BSP, Msanga HP. 2002. Dormancy and germination. In: Tropical tree seed manual, ed. J.A. Vozzo. U.S. Department of Agriculture Forest Science pp. 149-176.

Taiwo O, Xu HX, Lee SE. 1999. Antibacterial activities of extracts from Nigerian chewing sticks. Phytotherapy Research 13(8), 675-679.

Tchatat M. 1999. Produits forestiers autres que le bois d’œuvre (PFAB): place dans l’aménagement durable des forêts denses humides d’Afrique centrale. Série FORAFRI, document 18. CIRAD, CIFOR, IRAD & CARPE 103 pp.

Vogel E. de F. 1980. Seedlings of dicotyledons – Structure, development, types. Descriptions of 150 woody Malesian taxa. Centre for Agricultural Publishing and Documentation. Wageningen, 1980. ISBN 90‐220‐0696‐4.

Watson L, Dallwitz MJ. 2009. The families of flowering plants. Guttiferae. Juss. descriptions, illustrations, identification and information retrieval. Version 19th August 2014. http://delta-intkey.com.

Wong JLG. 1997. That State of Ghana’s Forest (1986-1997). Non-timber forest products. Fountain Renewable Resource Ltd., UK 29 pp.

Article source : Germination of Garcinia kola (heckel) seeds in response to seed sectioning, chemical pretreatment and different temperatures  

0 comments: