Turmeric Extract Effects on Rat Ovary and Uterus | InformativeBD

Efe Endurance Ahama, Omewomano Peace Onyilo, Emmanuel Agbamu, Igho Emmanuel Odokuma , Ijeoma Favour Ossai and Oroghene Clinton Ogorugba, from the different institute of the Nigeria. wrote a research article about, Turmeric Extract Effects on Rat Ovary and Uterus, entitled, Histologic sequelae following exposure to turmeric extract on wistar rats ovary and uterus.This research paper published by the International Journal of Biomolecules and Biomedicine | IJBB. an open access scholarly research journal on Biomedicine. under the affilition of the International Network For Natural Sciences | INNSpub. an open access multidisciplinary research journal publisher.

Abstract

The regular and single use of turmeric in reference to the reproductive system following several review is conflicting, and its effect has not been fully ascertained. The study therefore espoused the histologic sequelae following exposure to turmeric extract on wistar rats ovary and uterus. Ethical clearance was sought from the Research and Ethics Committee in the Faculty of Basic Medical Sciences of the Delta State University prior to the commencement of this research. 24 wistar rats divided into 4 groups of 6 rats each. Group A was the control group while groups B, C and D were the treated groups that received 500, 1000 and 1500mg/kg of turmeric extract respectively. Animals were also sub-sectioned, labeled accordingly into 7th, 14th and 21st days respectively for easy identification and treatment or administration. At the end of each experimental days, animals were weighed and euthanized by cervical dislocation. Ovaries and uterus were dissected and fixed in 10% formal saline solution, following regulated histologic procedures. Prepared Slides were viewed using a digital microscope. The micrographs obtained revealed deleterious effect on the histology and cytology of the ovary and uterus following treatment of turmeric. The observed histological distortions to the ovary and uterus have most likely arisen from repeated exposure to tumeric extract; this has proven turmeric to be an harmful substance to the female reproductive system especially women of child bearing age and the regular and continuous consumption of these agent should be regulated. 

 

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Introduction

Turmeric is a commonly used spice, popularly known as Curcuma as its active ingredient. It is rhizomatous and herbaceous plant use to attain certain health ameliorating effect in humans (Lestari and Indrayanto, 2014). It possesses antioxidant, anti-inflammatory, antimicrobial, anti-angiogenic and anticancer effects, which ascertain it therapeutic property (Ahmad et al., 2020; Bahrami et al., 2020). It can also boost immunocompetent cells and produce inflammatory cytokines (Moody et al., 2020). On the otherhand its mechanism of action remain unknown in respect to reproduction, but it is a known fact that tumeric is phytoestrogenic in nature (Bachmeiri et al., 2010). It’s widely consumed as a traditional medicine despite the lack of awareness of its safety or toxic effects especially to the reproductive system (Jeber and Tawfeek, 2012; Thakur et al., 2009; Ilyas et al., 2019).

Reproduction is a vital biological process for maintenance of species. However, exogenous substances such as herbs can be used to suppress or promote fertility (Harat et al., 2008). A study has proven the beneficial antioxidative effect of curcumin on the testis and ovaries (Jeber and Tawfeek, 2012). In the ovaries, turmeric promotes ovarian viability, folliculogenesis, fecundity and response to hormones. Some studies have also reveal curcumin to mediate cell cycle arrest in many different tumour cells through the regulation of several proteins and cascades (Park et al., 2002; Aggrawal et al., 2006). Jeber and Tawfeek in their study also affirmed to the protective effect of curcumin as against potassium dichromate induced ovary. The beneficial effect of turmeric on dysmenorrhea and endometriosis, as a therapeutic major by way of reducing endometrial cells lining is also documented in the uterus (Utami et al., 2020; Zhang et al., 2013)

Apart from the beneficial effects of curcumin on female reproduction, ominious effects such as decrease of gonadotropin hormones and inhibition of the estrous cycle have been documented (Thakur et al., 2009). Studies have also proven turmeric to be an infertility agent with discoveries showing its relaxant properties that reduces the level of oxytocin thereby reducing the contraction of the uterus which is not healthy for women of childbearing age (Ittipanichong et al., 2003; Manvizhi et al., 2020). Morealso, turmeric has been shown to cause degenerative features of the graafian follicles and germinal epithelium of the ovaries with presence of rudimentary corpus luteam cells (Ray et al., 2011). Considering the aforementioned treatise reviewed, with contracdictory (safety and toxic) effect of the use of turmeric on female reproduction which is not fully studied or ascertained coupled with scanty reports on the anatomic histoarchitecture of uterus and ovary following exposure to the single use of turmeric. The study therefore evaluated the histologic sequelae following exposure to turmeric extract on wistar rats ovary and uterus.

Reference

Aggarwal S, Ichikawa H, Takada Y, Sandur SK, Shishodia S, Aggarwal BB. 2006. Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and anti-apoptotic and metastatic gene products through suppression of IκBα kinase and Akt activation. Molecular Pharmacology 69, 195 – 206.

Ahama EE, Odokuma EI. 2022. Histomorphological Effects of Oral Nicotine Administration on the Testes of Adult Wistar Rats. Journal of Chemical Health Risks. DOI: 10,22034/ jchr.2022.1949064.1488

Ahmad RS, Hussain MB, Sultan MT, Arshad MS, Waheed M, Shariati MA, Plygun S, Hashempur MH. 2020. Biochemistry, Safety, pharmacological activities and clinical application of turmeric: A mechanistic review. Evidence Based complementary and Alternative Medicine 7656919

Bachmeier BE, Mirisola V, Romeo F, Generoso L, Esposito A, Dell’eva R, Blengio F, Killian PH, Albini A, Pfeffer U. 2010. Reference profile correlation reveals estrogen-like transcriptional activity of curcumin. Cellular Physiology and Biochemistry 26, 471 – 482.

Bahrami A, Sathyapalan T, Moallem SA, Sahebkar A. 2020. Counteracting arsenic toxicity: Curcumin to the rescue. Journal of Harzardous Material 400, 123160.

Emani B, Farzaneh S, Ghorani V, Boskabady MH. 2017. Relaxant effect of Curcuma longa on rat tracheal smooth muscle and its possible mechanisms. Pharmaceutical Biology 55(1), 2248 – 2258.

Eze-Steven PE. 2019. Histopathological Investigations of Curcuma longa (Turmeric) and Zingiber officinale (Ginger) on rats with Monosodium Glutamate-induced Leiomyoma 7(1), 9-15

Harat ZN, Sadeghi MR, Kamalinejad M, Eshraghian MR. 2008. Immobilization effect of Ruta graveolens L. on human sperm: A new hope for male contraception. International Journal of Ethnopharmacology 115, 36-41.

Ibraheem BO, Oluwagbenga OI, Tawa OO, Jacob OO, Babatunde A. 2018. Effects of organic turmeric on liver integrity and oxidative stress of the brain in rabbits exposed to ultraviolet radiation. International Journal of Environment, Agriculture and Biotechnology 3(3), 838 – 845

Ilyas S, Hutahaean S, Elimasni E, Panjaitan SRN. 2019. Effect of turmeric rhizome extract on the kidney histology of preeclampsia rats. Earth and Environmental Science 305, 012078.

Itthipanichong C, Ruangrungsi N, Kemsi W, Sawasdipanich A. 2003. Antipasmodic effects of curcuminoids on isolated guinea pigs ileum and rat uterus. Journal of the Medical Association of Thailand 299 – 309.

Jeber ZK, Tawfeek FK. 2012. Effect of turmeric oil in reproduction efficiency of immature female rats exposed to oxidative stress induced by potassium dichromate. Journal of Pharmacy and BioAllied Sciences 9-13.

Lestari MI, Indrayanto G. 2014. Curcumin. Profile of drug substances, Excipients and Related Methodology 39, 113-204.

Manvizhi S, Sabitha SV, Aniket K, Margaret SFX. 2020. Effect of curcumin on the contraction of isolated goat uterus. International Journal of Basic Clinical Pharmacology 10(1), 81.

Marchiani A, Rozzo C, Fadda A, Delogu G, Ruzza P. 2014. Curcumin and curcumin-like molecules from spice to drug. Current medicinal Chemistry 21, 204 – 222.

Mohammed A, Wudil AM, Alhassan AJ, Muhammad IU, Idi A, Abdulmumin Y. 2016. Acute and sub chronic toxicity studies of aqueous, methanolic and n-Hexane root extracts of Curcuma longa L. on albino rats. British Journal of Pharmaceutical Research 14(2), 1 – 8.

Moody R, Wilson K, Jaworowski A, Plebanski M. 2020. Natural compounds with potential to modulate cancer therapies and self-reactive immune cells. Cancers (Basel) 12, 673

Naz RK. 2011. Can Curcumin Provide an Ideal Contraceptive? Molecular Reproduction & Development 78, 116 – 123

Park MJ, Kim EH, Park IC, Lee HC, Woo SH, Lee JY, Hong YJ, Rhee CH, Choi SH, Shim BS, Lee SH, Hong SI. 2002. Curcumin inhibits cell cycle progression of immortalized human umbilical vein endothelial (ECV304) cells by up-regulating cyclin-dependent kinase inhibitor, p21WAF1/CIP1, p27KIP1 and p53. International Journal of Oncology 21, 379 – 384.

Raj A, Singh A, Sharma A, Singh N, Kumar P Bhatia V. 2011. Antifertility activity of medicinal plants on reproductive system of female rat. International Journal of Bio-Engineering Science and Technology 2, 44 – 50.

Rauf M, Osman C, Sevil E, Asli C, Nese B. 2018. Beneficial effects of curcumin and capsaicin on cyclophosphamide-induced premature ovarian failure in a rat model. Journal of Ovarian Research 11(33).

Reddy PS, Begum N, Mutha S, Bakshi V. 2016. Beneficial effect of curcumin in Letrozole induced polycystic ovary syndrome. Asian Pacific Journal of Reproduction 5(2), 116 – 122.

Thakur S, Bawara B, Dubey A, Nandini D, Chauhan NS, Saraf DK. 2009. Effect of Carum carvi and Curcuma longa on hormonal and reproductive parameter of female rats. Internaional Journal Phytomedicine 1, 31 – 38.

Utami RB, Damayanti DF, Rodiah D. 2020. The effectiveness of Curcuma longa drink in decreasing the intensity of Dysmenorrhea. Biomedical and Phamacology Journal 13(4), 2055 – 2060

Wang X, Zheng M, Liu J, Huang Z, Bai Y, Ren Z, Wang Z, Tian Y, Qiao Z, Liu W, Feng F. 2017. Differences of first pass effect in the liver and intestine contribute to the stereoselective pharmacokinetics of rhynchophylline and isorhynchophylline epimers in rats. Journal of Ethnopharmacology 209, 175 – 183.

Yan F, LaMarre JM, Rohrich R, Wiesner J, Jomaa H, Mankin AS, Fujimori DG. 2010. RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA. Journal of the American Chemical Society 132, 3953 – 3964.

Yan Z, Dai Y, Fu H, Zheng Y, Bao D, Yin Y, Chen Q, Nie X, Hao Q, Hou D, Cul Y. 2018. Curcumin exerts a protective effects against premature ovarian failure in mice. Journal of Molecular Endocrinology 60(3), 261 – 271.

Zhang Y, Cao H, Yu Z, Peng H, Zhang C. 2013. Curcumin inhibits endometrial cells by reducing estradiol production. Iranian Jouranl of Reproducive Medicine 11(5), 415 – 422.

 Source : Histologic sequelae following exposure to turmeric extract on wistar rats ovary and uterus