Cell Cycle Insights: Comparing Mitoticages in Bangladeshi Brassica Varieties St | InformativeBD

Susmita Saha, from the institute of Bangladesh and Kazi Nahida Begum, from the institute of Bangladesh. wrote a Research article about, Cell Cycle Insights: Comparing Mitoticages in Bangladeshi Brassica Varieties St. entitled, A comparative analysis on mitotic interphase and prophase among twelve varieties of Brassica L. from Bangladesh: Brassicaceae. This research paper published by the International Journal of Biosciences | IJB. an open access scholarly research journal on Biosciences. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

Brassica L. is an agronomical and economical important crop belonging to Brassicaceae family. The research was conducted to interrelate interphase nuclei and prophase chromosome of Brassica L. varieties. In the current analysis, the nature of interphase nuclei and prophase chromosome of twelve BARI varieties of Brassica L. was investigated based on orcein-staining properties. In the interphase nuclei only ‘Diffuse type’ and ‘Simple Chromocenter type’ were found in Tori-7, Dawlat, BS-11, BS-14 and SS-75, Rai-5, BS-6, BS-7, BS-8, BS-10, BS-12, BS-15, respectively. Considering prophase chromosome among these studied twelve varieties, ‘Continuous type’ showed in Dawlat, Rai-5, BS-7, Bs-8, BS-11, BS-14 while BS-6, BS-10, BS-12 and BS-15 observed ‘Interstitial type’ and ‘Gradient type’ showed in Tori-7, and SS-75. Therefore, the orcein staining property of interphase nuclei and prophase chromosome can provide essential information as cytological implement to discriminate the twelve analyzed Brassica L. varieties.

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Introduction

Brassica L. is one of the most commercially important genus of Brassicaceae family which wildly distributed throughout the world. This genus mainly originated from close regions of Himalaya and then dispersed from Asia to European-Mediterranean territory (Downey and Robelen, 1989). About contrasting 37 species includes into the genus Brassica L. with divergent agronomic traits as vegetable and oilseed crops (Jahan et al., 2013). On the basis of divergent morphological characteristics and genetic diversity six interlinked species are found in the genus Brassica L., of which three amphidiploids species (B. carinata, B. juncea and B. napus) are evaluated from three diploid species, (Brassica campestris, B. nigra, and B. oleracea) (Nagahara, 1935). Among these six varieties, the four most widely cultivated species are Brassica campestris L., B. juncea (L.) Czern and Coss., B. napus L., and B. carinata Braun for oilseed and vegetables (Rakow, 2004).

Generally, the genus Brassica L. can be categorized into three groups: rapeseed, mustard and cole. Rapeseed-mustard is well known for edible oil and protein whereas cole is consumed as vegetables. As oil seed Brassica L. achieves second largest contributor role in global oil production after soybean (Raymer, 2002). Mustard oil is not only used for as cooking oil but also marinate food stuffs and salad dressings. Moreover, as an edible oil Brassica L. is worthier for human health due to the presence of linoleic acid (omega 3 fatty acid) and alpha linoleic acid (omega 3 fatty acid) (Mollika et al., 2011).

Brassica L. is an excellent source of potassium, phenolics, dietary fiber, vitamins A, C and E as well as a renewable resource in the petro-chemical industry (Zhang et al., 2006).

The oil meal of Brassica L. has a quality value in beef and poultry ration as a protein supplement. According to Luciano and Holley (2009), the mustard has antibacterial and antifungal properties considering the substance similar to glucosinolate.

Brassinosteroids have a great influence to control both prostate and breast cancer which are highly distributed in pollen and seed of B. campestris and B. napus (Wachsman et al., 2012).

Due to the commercial values of Brassica L., a large number of tremendous investigations have been occurred for morphological, physiological and biochemical improvement in worldwide as well as Bangladesh (Zhang, 1996; Chen et al., 2001; Hu et al., 2001; Khan et al., 2002; Xiao et al., 2004; Liu, 2007; Mollika et al., 2011; Akbar and Begum, 2020; Paul et al., 2020). But still it is essential to know the cytological and cytogenetical information of a species because it plays a significant role to relevant its evolution and diversification (Ropiquet et al., 2008). Based on previous literature, researchers were concentrating to reveal the chromosomal information and molecular analysis of Brassica L. throughout the world (Takamine, 1916; Du et al., 1993; Olin-Fatih 1994; Cheng et al., 1995; Fukui et al., 1998; Kulak et al., 2002; Snowdon 2007; Fang et al., 2014; Sun et al., 2018). On account of small size chromosome, only chromosome number and classical cytogenetical information is insufficient for characterization of species or varieties (Begum and Alam, 2016). In such cases, the feature of interphase nuclei and prophase chromosome act as key cytological tool to characterization of any specimens. Therefore, the current investigation has been approached to reveal the nature of interphase nuclei and prophase chromosome of mitotic cell division using orcein staining that should provide convenient information for disguising twelve BARI varieties of Brassica L., because this information is not available in the contemporary scientific literatures.

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Article source : A comparative analysis on mitotic interphase and prophase among twelve varieties of Brassica L. from Bangladesh: Brassicaceae