DNA Uncovers Forest Fungi: Identifying Wild Mushrooms from Mt. Mingan | InformativeBD

Chenny Dhanryll M. Guzman, Marisol M. Baltazar, Aiza Jhane I. Sanchez, Mariel G. Linsangan, and Rich Milton R. Dulay, from the different institute of Philippines, wrote a Research article about, DNA Uncovers Forest Fungi: Identifying Wild Mushrooms from Mt. Mingan. Entitled, Molecular identification of four wild higher basidiomycetes collected in Mt. Mingan, Gabaldon, Nueva Ecija, Philippines. This research paper published by the Journal of Biodiversity and Environmental Sciences |JBES. an open access scholarly research journal on Biodiversity. under the affiliation of the International Network For Natural Sciences| INNSpub. an open access multidisciplinary research journal publisher.

Abstract

This paper reports the molecular identification of the four mushrooms collected in Mt. Mingan, Gabaldon, Nueva Ecija, Philippines. The DNA samples from the fresh fruiting bodies of mushrooms were extracted, isolated, purified, PCR amplified (using ITS-1F and ITS4 primers), and sequenced (using Capillary Electrophoresis Sequencing). The DNA sequences were trimmed and cleaned-up before sequence alignment using MEGA7 software and the phylogenetic affiliations of the ITS genes were determined by alignment to the NCBI non-redundant database using the BLASTn algorithm. The four mushrooms were identified as Stereum hirsutum (95%), Micropus xanthopus (99%), Pleurotus tuberregium (99%) and Trametes elegans (99%). To our knowledge, this is the first report on the collection and molecular identification of these wild mushrooms in Mt. Mingan.

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Introduction

Mushrooms are basidiomycetous fungi which are naturally found growing on lignin-cellulosic substrates in the temperate and tropical regions. This group has been reported to be beneficial to humans. Wild mushrooms are seasonally and traditionally collected in the forest and/or any areas with lignincellulosic substrates and consumed by farmers and mushroom hunters. This is due to their nutritional as well as their therapeutic values (Rahi and Malik, 2016). They contain proteins, fibers, carbohydrates, and minerals (Amabye and Bezabh, 2015) and use for the development of medicines, pharmaceutical products such as drugs, dietary supplements, and healthy cosmetic products (Tang, 2004).

The Philippines, being one of the mega diverse countries in the world, is rich in mycological resources that remain in the wild and underutilized. Several studies have been conducted on the taxonomic identification and listing of wild macrofungi in the Philippines. For instance, in Mt. Malinao, Albay, 9 Tricholomataceae, 3 Coprinaceae, 2 Pluteaceae, and 1 Auriculariaceae species were identified (Daep and Cajuday, 2003) while Mt. Apo in Mindanao had 25 genera and 87 species of basidiomycetes (Biadnes and Tangonan, 2008). Moreover, Militante and Tadiosa (2005) identified 27 different wood-decaying species of Basidiomycetes belonging to 15 genera whereas 30 species classified under 18 families and 21 genera were listedin agroecosystem of Brgy. Bambanaba, Cuyapo, Nueva Ecija (Dulay and Maglasang, 2017).

However, identification and classification of these wild mushrooms are basically morphological which is sometimes leading to misidentification of the species. Therefore, it is indeed necessary to use molecular approach in order to have accurate and reliable fungal taxonomic studies.

This present work molecularly identified the four wild mushrooms collected in Mt. Mingan, Gabaldon, Nueva Ecija, Philippines.

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Article source : Molecular identification of four wild higher basidiomycetes collected in Mt. Mingan,Gabaldon, Nueva Ecija, Philippines