Naseem Khatoon Bhurgri,
from the institute of Pakistan. wrote a Research article about, Starting
Strong: Optimizing Micropropagation Protocols for Sugarcane US-718. Entitled, Initiation
protocol optimization of (Saccharum officinarum L.) variety US- 718 through
micropropagtion techniques. This research paper published by the Journal of Biodiversity and Environmental Sciences | JBES. an open access scholarly
research journal on Biodiversity. under the affiliation of the International
Network For Natural Sciences| INNSpub. an open access multidisciplinary
research journal publisher.
Abstract
The sugarcane varietyUS-718 experimentally was carried out in changed concentration of BAP and
kinetin under established condition of invitro along with
replications in complete randomized design. The purpose of the study was to
determine the optimum concentration in presence of combination of BAP and
kinetin under invitro condition .In MS neutering media the variety
US-718 shown maximum result of 90.32% shoot initiation in 3.5#0.5 of shoots per
explant in concentrations of 1.5mg/l BAP without kinetin exhibited a better
response than other concentration.
Read more : Fruit Tree Biodiversity: Endemic and Introduced Species in Samboja District |InformativeBD
Introduction
Sugarcane belong to
Poaceae family is a strong and major crop of Pakistan. It consists high sugar
content. (Saccharum officinarum) contributes 60-70% of annual sugar production
in the world (Shakra Jamil 2017). Sugarcane is a perennial grass family crops
that reproduction through sexual and asexual modes. It reproduces asexually by
three or two buds stem cutting called sets, and advanced methodologies of in
vitro propagation by taking parts of it such as shoot tip, apical merited, axillary
bud and leaf. It also reproduces through seed propagation via flower (Fuzz),
which is used for breeding purposes (Sime M2013). The sugarcane is used in
production of paper, antibiotics, dextran, waxes and fats, epoxy polymers and
bio fertilizers as reported by DSD, 2013. FAO 2014 .The sugarcane is
economically vital industrial crop and well propagated conservatively by sets,
but enormous land requirement, potential transfers of pathogens through seed
and conventional method from generation to generation are the main factors for
low rate propagation The invitro propagation is a greatest substitute to
overcome such factors and pave the way to produce disease free and enough
amount of planting material. (Belet Getnet 2017).
Clonal propagation
through tissue culture is the one of the extensively accepted methods used for
the commercial production of genetically enhanced quality of crops (Tiwari et
al., 2012). During tissue culture, pathogenic and non-pathogenic fungi and
bacteria are exposed as contaminants and therefore can regularly be disposed
of. Systemic viruses which are much more difficult to eliminate the sugarcane
intensive persistent contamination has been frequently reported in many tissue
culture system, despite, the use of strong surface sterilization treatments of
explants (Anonymous. 2009). Micropropagtion is however actual subtle methods
which needs sterile condition in each stage (0-4) specifically for
establishment of surface sterilization of explants initiation and establishment
of aseptic culture, multiplication, rooting and acclimatization. These steps
manifested the sensitivity of tissue cultures techniques in determination of
contamination and economic significance rather than conventional propagation.
These techniques facilitates to grower to permit the sugar industry estates to
produce adequate planting material with short time period and cost effective
(Lakshman p. et al., 2006).
Shoot tip initiation
observed at different concentration by BAP and Kin in many plant culture
systems (G. Zahra et al., 2010). The success of the sugarcane tissue culture is
also dependent of sugarcane varieties. (C. Ganonou et al., 2005), the tissue
culturing medium and use of growth enhancer and regulators (V. Saharan et al
2004). Therefor the present study on sugarcane variety US-718 was carried out
by plant tissue culture laboratory Khoski Sugar mill Badin, to detect the
influence of dissimilar media compositions on the initiation of sugarcane
variety US-718. We have also carried out optimization of initiation protocol
for in vitro propagation of sugarcane variety US-718.
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